Effect Of Modified Decoction On Behavioral Symptoms And NO,NOS And CGRP Contents In Migraine Model Rats Induced By Nitroglycerin

Object:To investigate the effects of different doses soup flavored bulk under partial nitroglycerin induced migraine model of behavior.Study of nitroglycerin-induced migraine big plasma of NO,NOS,CGRP and spinal trigeminal nucleus NOS₁,CGRP Contents.To investigate the analgesic effect of Modified Partial soup scattered and Mechanism.Provide a solid theoretical basis for partial Modified Powder Treated Migraine Disease.Method:1.The rats were divided into control group,model group,the bulk partial soup flavored high,medium and low dose group,sulbactam group,San soup flavored with partial high,medium and low dose(35.64,17.82,8.91g· kg^-1 · d^-1)Continuous gavage 5 days?Nitroglycerin-induced migraine rats,30 min after the last administration and the control group with 95% ethanol 2 mlg· kg^-1 subcutaneous outside.Other groups inject nitroglycerin 10mg·kg^-1.Copy he rat model of migraine.2.Determination of model behavior:Red Ea-To observe the time of appearance and disappearance of the red ear rats after modeling;Scratching-From the time of modeling from observing times of each time period scratchingthe model rats modeling from the beginning to the duration of the segment counting method every 30 minutes as a time period.Observing for 5 continuous perio.The total number of scratching were recorded four time periods3.Plasma NO,NOS and CGRP Level:Subcutaneous injection of nitroglycerin injection after 4h and then anesthesi.Abdominal aortic blood with a syringe,Plasma was separated determination of CGRP and NO,NOS level which in strict accordance with the detection of plasma CGRP radioimmunoassay kit Operating instructions.NO and NOS 1 levels measured in the visible light spectrophotometer a ccording to kit instructions to operate4.Spinal trigeminal nucleus NOS₁,detecting CGRP Expression: The blood of rat aortic cannulation,cut the right atrial appendage,poured into 4% paraformaldehyde in 0.1 mol / LPBS liver to harden,still to limbs.Remove the brainstem and the medulla into rat tail section formaldehyde solution overnight.After paraffin-embedded rat brainstem and medulla tail section,continuous coronal sections,and we take one every five pieces.Slice dewaxing to water,citric acid antigen retrieval,slice and then add antibody.Each slice NOS₁ observed at 10 × objective lens,CGRP,positive cells,Germany Lecia photomicrograph system with image acquisition and analysis,fluorescence microscopy showed positive red cells,used to record the number of positive cells in the image.Results:1.Influence of disappearance and migraine time of rats had red ears.Blank red ear group of symptoms does not appear,the remaining rats were about 10 min appear red ear symptoms.Comparing with the model group,high bulk partial soup flavored,middle dose group can significantly slow down the time of occurrence of red ears.Shu Mapu Tan group scattered partial soup flavored high,medium and low dose group can significantly shorten the time of the disappearance of rat red ear?P<0.01?,and the middle dose group show more significant differences.2.Nitroglycerin-induced migraine number of rats scratching their heads.After injection of nitroglycerin modeling within 30 min in each group of rats scratching frequency was significantly increased compared with the control group?P<0.01?,and each group had no significant difference in the number of scratching their heads,indicating successful model.After administration 60 min,flavored bulk partial soup high in comparison,scratching times were significantly reduced low-dose group and model group?P<0.01?,compared with the model group,Jiawei bulk partial soup of high,medium dose group with the high dose the strongest group?P<0.01?3.Modified Powder partial soup migraine rat plasma,NO,NOS,CGRP contents.After modeling,rat plasma NO,NOS,CGRP increased significantly with the control group?P<0.01?,the difference was statistically significant.Compared with the model group,the bulk partial soup flavored high,medium and low dose group inhibited to varying degrees of NO,NOS,CGRP increased?P<0.01?,compared with the model group,the bulk partial soup flavored with highdose group significantly?P<0.01?4.Flavored soup of San partial spinal trigeminal nucleus NOS₁.Jiawei bulk partial soup can affect the number of cells the expression of CGRP.Compared with the control group,immun-ofluorescence staining was found in the model group spinal trigeminal nucleus NOS₁ and CGRP-positive cells was significantly increased?P<0.05,P<0.01?than the control group,the bulk partial soup flavored high,medium and lowdose group than in the model group,the spinal trigeminal nucleus NOS₁ significantly reduced and CGRP-positive cells?P<0.01?Conclusion:1.Modified Powder partial soup can effectively reduce migraine ear red rat disappeared time,reduce the number of migraine scratching rats which suggesting that this can only effectively relieve migraine symptoms in rats.2.Modified Powder partial soup can reduce migraine rat model of plasma NO,NOS,CGRP contents and inhibits spinal trigeminal nucleus and CGRP NOS₁ neurotransmitter activation,thus combating vasoactive substances and neurotransmitter disorders,regulating brainand disorders of vascular function.