The Correlation Research Between D-IBS Patients With The Changes Of Intestinal Mucosal Advantage Bacterium And The Expressions Of TLR2 And TLR4

Objective:To research the changes of Diarrhea-predominant Irritable Bowel Syndrome(D-IBS)patients with intestinal mucosal dominant bacterium and its mechanism related to TLR2 and TLR4,so that to provide a useful reference for further exploring the pathogenesis of IBS.Methods:By colonoscopy collected descending colon mucosa tissue of 20 cases of D-IBS patients and 20 cases of normal controls as the experimental group and control group respectively,in order to understand the characteristics of the intestinal mucosal flora and select dominant bacterium,high-throughput sequencing of 16 SrRNA V3-V4 area were used on these specimens,and then it is that the quantities of the Bacteroidetes,Firmicutes and Clostridium three kinds of dominant bacterium and the expressions of TLR2 and TLR4 mRNA in the mucosa of D-IBS patients and normal controls were detected by reverse transcription PCR and quantitative real-time PCR respectively.So that to analyze the characteristic of three kinds of dominant bacterium changes in the mucosa and the relationship with the expressions of TLR2 and TLR4.Results:1.Compared with those in control group,the flora diversity,Bacteroidetes and Clostridium in the mucosa were significantly reduced in D-IBS group(P<0.05),while thequantities of Firmicutes in the mucosa did not show significant differences(P>0.05);2.Compared with those in control group,the expressions of TLR2 and TLR4 were all significantly increased in D-IBS group(P<0.05),and there was a positive correlation between TLR2 and TLR4(P<0.01);3.The correlation study in the D-IBS group found,the Bacteroidetes have a negative correlation with the expressions of TLR2 and TLR4(P<0.01);Clostridium has a negative correlation with TLR2(P<0.01),but dose not with TLR4(P>0.05);while the intestinal mucosa flora and TLR showed no correlation in the control group(P>0.05).Conclusion:1. In D-IBS patients,there not only have flora imbalance and abnormal expression of TLR in the mucosa,but have correlation with each other.2.The flora imbalance may be correlation with the pathway of TLR in the occurrence of D-IBS.