Effects Of Endothelial Function On Rabbit Atherosclerotic Vulnerableplaque Model With Eerdun-wurili

Objective By observing the amount of Eerdun-wurili Rabbits atherosclerotic vulnerable plaque model of vascular endothelial growth factor ET, PAF, TXA-2, PGI2, VEGF, E-selectin, SDF-1 and vascular endothelial coagulation fiber soluble substance t-PA, PAI-1, Fbg influence its clear plaque stabilization mechanism.Methods 50 rabbits were randomly divided into normal group and model group. Normal group 10 without modeling, normal diet; model group 40 high fat diet, immune injury, balloon injury factor complex modeling technique to establish rabbit atherosclerotic vulnerable plaque model. 8 weeks after the model is successful, the model group were randomly divided into model group, simvastatin group, the amount of Eerdun-wurili group, the normal group and model group were fed with the same volume of distilled water, the capacity of the administration are 4ml · kg-1 · d-1; simvastatin group received simvastatin 5mg · kg-1 · d-1; scale group given the amount of Eerdun-wurili 0.4g · kg-1 · d-1, continuous administration for 16 weeks. 24 weekend end of the experiment, the final completion of the experiment 38 rabbits(normal group=10, model group=10, Eerdun-wurili group=10, simvastatin group=9) were sacrificed. Observing the following indicators:1.using immunohistochemistry to detect the rabbit aortic vascular endothelial growth factor ET, PAF, VEGF, SDF-1, t-PA, the expression of PAI. 2. Elisa method to detect the use of rabbit serum vascular endothelial growth factor ET, PAF, TXA-2, PGI2, VEGF, E-selectin, SDF-1, t-PA, PAI, Fbg concentration.Results 1 immunohistochemistry results showed that:(1) the amount of Eerdun-wurili group can lower blood ET, PAF expression(P <0.01), a decrease in the expression of ET than simvastatin group(P <0.05).(2) Compared with model group, the amount of Eerdun-wurili aorta group significantly reduced the expression of VEGF and SDF-1, but the impact of E-selectin is not significant.(3) Compared with the model group, the amount of Eerdun-wurili aorta group significantly decreased expression of PAI-1(P <0.01), and the expression level lower than the simvastatin group(P <0.05), but no significant difference(P> 0.05) affect the t-PA. 2.Elisa test results showed that:(1) Compared with the model group, Eerdun-wurili group significantly decreased serum ET, PAF(P <0.01), is superior in reducing the level of ET on simvastatin group.(2) Eerdun-wurili group significantly decreased serum TXA-2, and elevated levels of PGI2.(3)Eerdun-wurili group significantly reduce rabbit serum VEGF and SDF-1 concentrations(P <0.01), and it reduces the concentration of SDF-1 is superior to simvastatin group(P <0.05).(4) Compared with the model group, Eerdun-wurili group rabbits and Fbg serum PAI-1 levels were significantly lower(P <0.01), but no significant difference(P > 0.05);Conclusion Eerdun-wurili having improved atherosclerotic vascular endothelial function,which may reduce the vascular endothelial growth factor by ET, PAF, TXA-2, VEGF, SDF-1 levels and increased serum levels of PGI2; but also to exert anti-AS and stabilizing vulnerable plaque by reducing the role of serum vascular endothelial coagulation and fibrinolysis substance PAI-1, Fbg level...