Analysis Of The Association Between Polymorphism Of ATP2B1 Gene With Genetic Susceptibility To Hypertension In Metabolic Syndrome Patients Of She Population In Fujian Province

Objectives: Essential hypertension and metabolic syndrome were the major noninfectious diseases caused by the interaction with a variety of genes and various environmental factors.The cardiovascular risk of metabolic syndrome patients with hypertension was significantly increased. She population was one of the major ethnic minorities along the southeast coast, and the relevant research of She population was rare currently. Therefore, the present research selected the metabolic syndrome patients from She population, to probe the relation between the polymorphism of rs2681472 loci of risk gene ATP2B1 and genetic susceptibility to hypertension, to analysis the association between fetal exposure to famine and blood pressure level, and to explore the interaction of gene and fetal exposure to famine impacting essential hypertension.Methods: We chose a case-control design methods, and stratified random sampling from 20-80 years old unrelated She population of long-term residents in Ningde city who were diagnosed metabolic syndrome, amount to 781 subjects. Thereinto, all participants were divided into 215 males and 566 females; 575 participants were classified as hypertensive patients and the others were without hypertension; 83 participants undergoes fetal exposure to the famine and 314 participants were not exposed. We gathered anthropometric data and clinical biochemical indexes, and drew peripheral blood leukocytes for extracting DNA. Relying on Taqman probe, genotyping was performed on Real-time PCR(ABI 7500)system. Data was conducted by Windows SPSS 19 statistical software package.Results: Among the metabolic syndrome patients in She population, AG, GG genotype and G allele frequency of hypertension group was more than nonhypertensive group in risk gene ATP2B1 rs2681472 loci. The risk of carriers with risk allele G was 1.291 times more than carriers with A allele(OR=1.291,95%CI:1.212-1.632,χ2= 4.548,P= 0.033). Among different genotype of AA,AG,GG, the prevalence of hypertension increased sequentially. Logistic regression model showed, after the adjustment of age, sex, BMI, height, weight, waist circumference, TG, HDL-C, TC, LDL-C, FPG, OGTT-2h PG, smoking and alcohol consumption, in the additive model: AG genotype significantly increased the risk of hypertension(OR= 1.923,CI: 1.278-2.894,P=0.002); GG genotype increased the risk of high blood pressure(OR=2.056,CI: 1.028-4.110,P=0.041);GG genotype also positively associated with SBP(OR= 2.132,CI: 1.103-4.119,P=0.041).In the dominant model: AG + GG significantly increased risk of hypertension(OR= 1.943,CI: 1.308-2.886,P=0.001);and it also associated with the SBP(OR= 1.533,CI: 1.056-2.226,P=0.025).(2)We used non-conditional Logistic regression analysis, adjusted for the factors of age, sex and BMI, and found that: the risk of the subjects who had gone through the famine fetal exposure significantly increased on developing into high blood pressure(OR= 2.109,CI:1.249-3.561,P= 0.005); and it also positively associated with SBP(OR= 2.420,CI:1.453-4.031,P= 0.001).(3)We used non-conditional Logistic regression analysis, after the correction of age, sex, BMI, height, weight, waist circumference, TG, HDL-C, TC, LDL-C, FPG, OGTT-2h PG, smoking and alcohol consumption, there was no significant association on the interaction of rs2681472 loci polymorphism and fetal exposure to famine to hypertension prevalence(P>0.05). Metabolic syndrome patients in the presence of fetal exposure to famine, the risk of risk allele G carriers suffering from hypertension was 2.187 times more than those without allele G(OR=2.187,CI:1.157-4.135,P=0.015).Conclusion: Among the metabolic syndrome patients, the polymorphism of ATP2B1 gene rs2681472 loci increased the susceptibility to hypertension. Carrying risk allele G significantly increased the risk of high blood pressure, and positively correlated with SBP. Fetal exposure to famine significantly increased the risk of hypertension, and also positively associated with SBP. Fetal exposure to famine was a independent risk factor for metabolic syndrome patients suffering from hypertension. There was no significant association on the interaction of rs2681472 loci polymorphism and fetal exposure to famine to metabolic syndrome patients suffering from hypertension. In the presence of fetal exposure to famine, carrying the risk of allele G had a greater risk than those without the allele G on suffering high blood pressure.