Effect Of Nuclear Factor NF-KB On The Level Of Apoptosis And Autophagy In Benign Prostatic Hyperplasia

Objective:To explore the effect of nuclear factor kappa B(NF- kappa B, NF-κB) on the benign prostatic hyperplasia(BPH) cell line. NF-κB activator and inhibitors were used to create different groups to observe the effect of NF-κB on autophagy and apoptosis on prostatic hyperplasia epithelial cells. In addition, the relationship between apoptosis and autophagy can be inferred from this experiment.Methods: Normal prostatic hyperplasia epithelial cells cultured in vitro as control group. NF- kappa B low activity group was established through PDTC inhibiting the activation of NF- kappa B protein and blocking its entry into the cell nucleus to exerting its biological function. NF- kappa B high activity group was established through PKC pathway which can activate nuclear factor kappa B. Western‐blot assay was done to detect the amount of nuclear factor NF- kappa P65 in each group. Cell apoptosis was detected by flow cytometry and Annexin V‐ FITC/PI staining. Western‐blot assay was used to detect the expression of apoptosis level related protein Bcl-2, Caspase-3, PARP-1 and autophagy level protein LC-3 in each experimental group.Results:Compared with the control group, the amount of NF-κB P65 in the nucleus of PDTC group was significantly decreased, while, the amount of NF-κB P65 in the nucleus of PMA group was significantly elevated. All the data were statistically significant(P<0.05). The level of cell apoptosis in PDTC group was significantly increased. There was no significant difference between the PMA group and the control group. The expression level of Bcl-2 protein in PDTC group was significantly lower, while the expression of Caspase-3 protein was significantly increased(P<0.05). The degree of cleavage of PARP-1 protein in PMA group decreased gradually over time. The autophagy level protein LC-3 II was significantly decreased in PMA group(P<0.05).Conclusions: PDTC and PMA can regulate the amount of P65 protein in the cell nucleus. NF-κB has inhibitory effect on the level of apoptosis and autophagy in prostate hyperplasia cells. NF-κB plays a favorable role in promoting the life activity of prostate hyperplasia cells. The occurrence of benign prostatic hyperplasia may be related to the level of apoptosis and the changes of autophagy.