Research On The Relevance Of The Activation Of Locus Coeruleus And Immunosuppression After Stroke

Background:In recent years, the clinical treatment of stroke has got rapid development, the cure rate and the recovery rate of patients with stroke have improved significantly, but there are still some clinical problems left unsolved in stroke treatment, in these problems, stroke-associated infection, SAI has got people’s highly attention. Infection is the most common of patients with acute severe stroke, incidence rate is as high as 21% to 65%, in which is often common to see pneumonia and urinary tract infection, it is always difficult to control in clinical, often leads to rapid deterioration and to prevent stroke early control of primary health and functional recovery. Stroke related infections are thought to be the independent risk factors of disease progression and death, which have brought heavy burden to patients and their families, and have become the major and to be solved clinical problems in the current field of neurology. Currently, the mechanisms of SAI:in situ inflammation, HPA axis hypothesis, sympathetic pathway hypothesis, our previous studies have demonstrated that immunosuppression after stroke sympathetic pathway activation are relevant, but the origin of the sympathetic nervous system is locus coeruleus, the stress response can be activated in the locus coeruleus neurons, enhancing the synthesis and secretion of norepinephrine to participate wake and alert, but also to enhance prefrontal cognitive function, improve the motivation level, thus improving sympathetic activities. This paper studies the correlation between inhibition against major locus coeruleus activation after stroke immunity.Purpose:Deep understanding biological mechanism of infection after stroke will help to find the clinical effective and targeted treatment, eventually improve the overall prognosis of patients with cerebral apoplexy.Methods:1. Group:MCAO group (The middle cerebral artery occlusion model by line written), DSP-4+ MCAO group (Using the neurotoxin DSP-4 chemical damage to the brain locus coeruleus nuclear production, and then make the middle cerebral artery occlusion model), the control group.2.ELISA methods is applied to study the inflammation index analysis in serum of the control group、the DSP-4+MCAO group and the MCAO group:IL-10、TNF-α、INF-γ.3. Western-blot method is applied to study the protein expression of NF-κB in spleen of the control group、the DSP-4+MCAO group and the MCAO group.4. Data analysis using GraphPad Prism 5 and SPSS for the statistical analysis software, the application of Excel diagram, the indexes to mean±standard deviation (x±s) said, the mean comparisons between two groups of paired t test, multiple sets of data using analysis of variance between; All experiment repeated three times, in order to P< 0.05 for the difference was statistically significantResults:1.In the peripheral blood, the concentration of IL-10:the sham operation group (7.726± 0.8492ng/L), the DSP-4+MCAO group (8.153±0.8895 ng/L) and the MCAO group (10± 0.2034ng/L) had statistically significant difference in P value=0.0136. The serum level of TNF-α:the operation group (6.457±0.3743ng/L), the DSP-4+MCAO group (6.433 ± 0.7508 ng/L) and the MCAO group (5.140 ± 0.4986ng/L) had statistically significant difference in P=0.0011. INF-γ concentration:the sham operation group (7.177 ± 0.6336ng/L),the DSP-4+MCAO group(7.291 ±/-0.9511 ng/L) and the MCAO group (5.882±0.5820ng/L) had statistically significant difference in P=0.0068.2. In the spleen, NF-κB used protein expression. The expression of NF-κB protein expression was significantly reduced in cytokine protein in the spleen, the DSP-4+MCAO group had no significant difference compared with the sham operation group.Conclusions:1. Pro inflammatory cytokines decreased, rats in the MCAO group compared with the sham operation group in peripheral blood, anti-inflammatory factor increased significantly, the phenomenon of obvious immune activation appeared; while the rats in the DSP-4+ MCAO group compared with the sham operation group in the peripheral blood of proinflammatory cytokines, inflammatory factor concentration did not change significantly.2. The MCAO group compared with the sham operation group, the expression of NF-κB protein expression was significantly reduced in cytokine protein in the spleen, the DSP-4+MCAO group had no significant difference compared with the sham operation group.