Studied On Improvement Process Of Danhong Preparation,The Chemical Constituents And Biological Activities Of Zephyranthes Candida

There are two sections in the this dissertation. The first section is the process improvement of preparation and plasma concentration of Danhong, and the other section is study on the chemical constituents and biological activities of Zephyranthes candida.The oral preparation of Danhong is the complex prescription in the treatment of cardio-cerebrovascular diseasewere, which is made up of radix Salvia mitiorrhizae and flos Carthamus tincuorius extracts. Based on the preliminary study, Danshengsu can be absorbed well, while no absorption for Safflower yellow in Danhong oral preparation. Herein, aiming at the problem of absorption of SY, we did massive experiments,finally, we sifted out the suitable excipients and its dosage to greatly increase the absorption of HSYA in vivo. The plasma concentration of Danshensu and HSYA in rat were assayed by HPLC after administration. Blood drug concentration-time data after the administration were processed by the DAS2.1pharmacokinetic software, and the main pharmacokinetic parameters using SPSS software for statistical analysis. Pharmacokinetic results showed, the relative bioavailability was 149.17% for Danshensu,that was 72.57% for HSYA.Zephyranthes candida(Amaryllidaceae) is originated in the South America and cultivated as an ornamental plant in China. The bulbs have been used as a traditional medicine to treat diabetes, cancer and so on. So far, it’s rare to report about the chemical constituents and biological activities of Z. candida. In this dissertation, the chemical constituents and biological activities of the chloroform extract of the whole plants of Z. candida were studied, and 16 alkaloids were isolated, including two new alkaloids, 11β-hydroxylycoramine(1), 11β-hydroxylycoramin nitrogen oxide(2), and14 known alkaloids, 11β-hydroxygalanthamine(3), galanthamine(4), sanguinine(5),lycoramine(6), O-demethylycoramine(7), hemeanthamin(8), haemanthidine(9),tazettine(10), 5,6-dihydrobicolorine(11), trisphaeridine(12), N-trans-feruloyltyramine(13), N-cis-feruloyltyramine(14), O-demethylycorine(15), lycorine(16). Inaddition, all the compounds were measured the activity of anti-acetylcholinesterase and anti-inflammatory in vitro. The results showed that alkaloids 5 possesed significant anti-acetylcholinesterase activity with an IC50 value of 0.48 μM, and alkaloids 8 and 9 showed potential in vitro anti-inflammatory activity.