Primary Culture And Electrotaxis Of Cerebral Cortical Astrocytes From SD Rats And Tbi Patients In Vitro

Objective: Traumatic brain injury is a common disease in Department of Neurosurgery, which is characteristic of high disability rate and high fatality rate. After injured, patients often suffered motor and sensory disturbances, as well as changes of behavior and consciousness, which increased the burden of family and society. Method to reduce the high rate of disability caused by brain injury has received extensive attention, therefore CNS repair become research focus. Cell migration plays an important role in tissue repair has already been confirmed. Hence, effect of electric field on cell migration has great potential in the CNS repair. In this study, we aim to investigate the conditions of primary culture of cerebral cortical astrocytes in vitro and the effects of exogenous electric fields on cell morphology and migration.Methods: Choose cerebral cortical of SD rats and TBI patients to establish primary astrocytes culture in vitro. Offer the most suitable conditions to their culture and use them for electrotaxis research after purification and identification. Through the establishment of the blank control group and the electric field intensity gradient to identify the influences that electric field and its strength bring to astrocytes.Results: Cerebral cortical astrocytes from SD rats did not appear obviously morphological changes and show as random motion in blank control group, so were cells in the electric field, but the displacement speed of cell migration is faster than that of control group. Cerebral cortical astrocytes of TBI patients show as random motion in blank control group. But the electrotaxis of them is obvious, the cell morphology was realigned perpendicularly to the electric field direction, and cells migrated towards the anode. Meanwhile, the migration speed and directional trend of electricity are positively correlated to electric field strength.Conclusion: Under the action of a short time(≤4h) exogenous electric field, Cerebral cortical astrocytes from SD rats did not show obvious electrotaxis. Meanwhile, Cerebral cortical astrocytes of TBI patients show obviously realigned perpendicularly to the electric field direction and migrated towards the anode in the electric field, and the electrotaxis increased with the electric field strength. The same type cell of different species probability exist diverse electrotaxis mechanism.