Also In Cervical Microenvironment Square One Iron Protoporpphyrin Free Body Measurement And Clinical Value Of HPV Detection In The Early Cervical Cancer Screening

Objective To analyze the differences of positive detection rate and copy number of cell free ferrous protoporphyrin detection in cervical microenvironment、HPV DNA and E6/E7 mRNA between different progresses of cervical lesion, evaluating their clinical values in early screening of cervical cancer.Methods We collected cervical exfoliated cell samples from 199 women undergoing biopsy examination. All participants were divided into normal control, low-grade squamous intraepithelial lesions(LSILs), high-grade squamous intraepithelial lesions(HSILs), and squamous cell carcinoma(SCC) groups. In the application of molecular biology technology to detect cervical microenvironment also square one iron protoporphyrin free body,HPV DNA was tested with hybrid capture technology, and E6/E7 mRNA was detected with fluorescence quantitative hybridization. Immunohistochemistry was performed by detecting E6/E7 protein in all patients after surgery or cervical biopsy.Results 1.Combined results of FH detection and HPV E6/E7 mRNA detection demonstrated that the positive detection rates of the control group were lower than those of lesion groups at all levels(P<0.05). FH detection of high-level group and cervical invasive carcinoma group showed higher positive rates than that of the low-level group(P<0.05). There is no significant difference(P> 0.05) between the FH detection of high-level group and the invasive cervical cancer group.2.The copy number of high risk HPV E6/E7 mRNA significantly increased along with the aggravation of lesions(P<0.05), as statistical differences were found among the 4 groups(F=8.786). The proportion of patients with high risk HPV E6/E7 mRNA(copy numbers > 10,000) significantly increased in the high-level group and the cervical invasive carcinoma group. The positive rate of HPV E6/E7 mRNA in the control group was lower than those of lesion groups at all levels(P<0.05). The positive rates of HPV E6/E7 mRNA in high-level group and invasive cervical cancer group are higher than that of the low-level group(P<0.05). There was no statistical difference between the positive rates of HPV E6/E7 mRNA between the high-level group and the invasive cervical cancer group.3.The positive rate and copy number of HPV DNA in the control group were lower than lesion groups at all levels(P<0.01, F=2.324), whereas no statistical differences were found among the copy numbers of the low-level group, the high-level group and the cervical invasive carcinoma group. 4.Correlation: Correlation was found between the detection of HPV DNA and the FH detection(r=0.784); Significant correlation was found between the detection of HPV DNA and the detection of high risk HPV E6/E7 mRNA(r=0.816); Significant correlation was found between FH detection and the detection of high risk HPV E6/E7 mRNA(r=0.915).5.Immunohistochemical results show that the positive detection rate of control group was lower than lesion groups at all levels(P<0.05).5.Immunohistochemical results show that the control group were lower than don’t lesion group(P < 0.05) at all levels.Conclusion HPV infection is closely related to cervical abnormalities, persistent infection with the virus after cervical epithelial lesions occur, FH seepage quantity and HPV E6 / E7 mRNA expression and the copy number and is closely related to the degree of cervical lesions,Negative result of HPV DNA is very helpful to exclude the cervical abnormality, whereas positive detection of mRNA has great value in prediction of the disease. Combined the results of positive detection and copy number makes a comprehensive evaluation of the risk of cervical lesions. HPV E6/E7 mRNA detection is an effective method in early screening of cervical cancer.