Antimicrobial Effect Of The New Synthetic Peptide Inhibiting Streptococcus Gordonii On Titanium Implant

Background Dental implants have been applied to treat dentition defect more and more widely because of its own advantages. At present, titanium and titanium alloy is always used in the field of oral implant because of its good biocompatibility and its ability to form osseointegration. How to improve the success rate of implanting becomes a research hotspot in recent years. Study has showed that the bacteria colonization on the surface of the implant can form a bacterial biofilm, which leads to the occurrence of peri-implantitis, and ultimately leads to the failure of implant surgery. Existing studies has reported that the micro ecological environment around titanium implant is similar as natural teeth. In another way, Streptococci(such as Streptococcus gordonii, S. gordonii) is mainly the early colonization. Therefore, we try to use titanium inorganic binding peptide 1(min TBP-1) to connect the surface of the titanium with connecting molecules and antimicrobial peptides(AMPS)JH8194,which is the derivative of Histatins5, to inhibit the adhesion and growth of S.gordonii and prevent the formation of oral biofilm and the happen of inflammation.We use the inorganic binding peptide to immobilize the AMPs on the surface of titanium, which avoid the toxicity brought by covalent cross-linking. The application of AMPs may overcome drug resistance. This study may provide new thought for antibacterial activity on the surface of titanium implant.Objective To detect the independently designed synthetic peptide adsorbed to the titanium surface and its inhibitory effect on Streptococcus gordonii, and to provide a new means for antibiosis reseach on oral implants.Methods Certain physical and chemical properties of the synthetic peptide and antimicrobial peptide were measured by Ex PASy Prot Param tool, Prot Scale analysis,circular dichroism and Zeta potential instrument. The synthetic peptide was anchored on the surface of the titanium specimen through incubation at room temperature.Adsorption of the synthetic peptide to the titanium surface was examined through the X-ray photoelectron spectroscopy(XPS) and the atomic force microscope(AFM).The inhibitory effect on Streptococcus gordonii of the synthetic peptide fixed on the titanium surface was viewed through the confocal laser scanning microscopy(CLSM). The destructive effects of the synthetic peptide and the antimicrobial peptide on Streptococcus gordonii were observed through the transmission electron microscope(TEM).Results The independently designed synthetic peptide had still had the physical and chemical properties that the antimicrobial peptide desired. The synthetic peptide had already been detected on the titanium surface after incubated in a 5mg/m L synthetic peptide solution. The titanium specimen fixed with the synthetic peptide had inhibited the survival and adhesion of Streptococcus gordonii.Conclusion It is suggested that the independently designed synthetic peptide might have reached the goal of bacterial inhibition on the titanium surface.