A Preliminary Study About The Action Mechanism Of Zearalenone On The Influence Cell Proliferation In Endometrial Carcinoma Ishikawa Cells

Zearalenone(ZEA), also known as F-2 toxin, is one of class estrogen toxin produced by the scythe spore fungus. It is also the main members of the family about the creatures dihydroxy benzoic acid lactone(Resorcylic acid lactones, RALs). ZEA exist widely in plant crops such as corn, wheat. it make the test for ZEA is complicated. ZEA,as an exogenous estrogen sample mycotoxins, has high affinity with estrogen receptors. The reduction in the body and form hydroxylation products, which have larger proportion of alpha corn gibberellic enol(alpha Zearalenol, alpha ZEL) and less proportion of beta corn gibberellic enol(beta Zearalenol, beta ZEL). Alpha ZEL enhances estrogen effect significantly.Although there have a lot of research about ZEA toxicity and its mechanism of metabolites class the of action of estrogen and pathogenesis, ZEA is in the body’s absorption and metabolic pathways diversity, and it has interaction with other substances, ZEA mediated reproductive system more complex mechanism of action of target cells.In this study, endometrial carcinoma Ishikawa cells were regardedas the research object to investigate the cytotoxicity of ZEA and its possible mechanism.The effects of ZEA on Ishikawa cell morphological changes, cell proliferation, cell viability, cell cycle and cell molecular level control were detected by using the methods of CCK-8, DAPI staining, flow cytometry and q RT-PCR, the results :(1)The low concentration of ZEA(0.01~10μM) has no inhibitory effect on the proliferation of endometrial cancer Ishikawa cells, When the concentration of ZEA is 10 μM, there is some of the promoting effect for the proliferation of endometrial cancer Ishikawa cells in the 24 h, 48 h.(2)The high concentration of ZEA(30~100μM) have a significant inhibitory effect on the proliferation of endometrial cancer Ishikawa cells in 24 h, 48 h, 72h(p<0.05). the morphological changes of cells were obvious, and the shape became larger. Wall sticking ability enhancement, emergence of polyploid.(3)The high concentration of ZEA may inhibit cell proliferation by regulating the expression of P21, cyclin B1, Cdk1 and other related genes in the periodic signal pathway to induce the G2/M phase arrest.In conclusion, ZEA could be assumed that there are significant promote at lower doses and inhibitory at higher doses action to proliferation of Ishikawa cells(p<0.05), and block lies in G2/M phase(p<0.05).