Research On Syndrome Of Dampness In Middle Jiao Model Rats Affecting Aquaporins In Liver,Lung And Kidney By Pingwei Powder

Objective:Through the systematic study of Aquaporins distribution, quantitative, activity and controlling mode on Syndrome of dampness in middle Jiao model rats in liver, lung and kidney, exploring whether Syndrome of dampness in middle Jiao caused by influence on water channel protein, and Pingwei Power rely on adjusting the water channel protein expression of the distribution and regulate water metabolism, reveal the Syndrome of dampness in middle Jiao caused part of the molecular mechanism of cell water metabolism imbalance and Pingwei Power rely on targets of controlling across cell membrane water transfer.Method:Adopting the relatively mature 20 days of Syndrome of dampness in middle Jiao model,through the detection of rat gastric residual rate and the intestinal propulsion rate, selecting the method of phloroglucinol to detect the content of D-xylose, chemical colorimetry to detect the activity of Na~+-K~+-ATPase in liver, lung and kidney of rats, enzyme-linked immune competition law (ELISA) to detect the content and immunohistochemical SP method and the average optical density to detect expression of the distribution of AQP1, AQP4, AQP8 in liver, lung and kidney of rats.Results:1 Syndrome of dampness in middle Jiao model:①Through the general observation, model group rats significantly reduce the weight, food intake and drinking water, and poor mental state.②The objective index detection, gastric emptying disorder, content of serum D-xylose absorption and Na~+-k~+-ATP enzyme activity is reduced on the model group rats.③After treated by Pingwei Power,feeding, drinking water, weight, gastric residual rate, the intestinal propulsion rate, serum D-xylose absorption and Na~+-K~+-ATPase is on the mend.Speculated that the Syndrome of dampness in middle Jiao model rats building success.2 Proteins content in liver, lung and kidney of rats:After animal model building, model group rats Aquaporins protein content increased than in the blank group:AQP1, AQP4 and AQP8 in kidney;model group rats Aquaporins protein content reduced than in the blank group:AQP1, AQP4, AQP8 and Na~+-K~+-ATPase in liver, AQP1, AQP4, AQP8 and Na~+-K~+- ATPase in lung, Na~+ -K~+-ATPase in kidney.After the intervention by Pingwei Power, Pingwei Power group rats Aquaporins protein content increased than in the self-recovery group:AQP1, AQP4 and Na~+-K~+-ATPase in liver, AQP1, AQP4, AQP8 and Na~+-K~+-ATPase in lung, AQP1, AQP4, AQP8 and Na~+ -K~+- ATPase in kidney;Pingwei Power group rats Aquaporins protein content reduced than in the self-recovery group:AQP8 in liver.After untreated by the self-recovery, the self-recovery group rats Aquaporins protein content increased than in the model group:AQP1, AQP8 in liver, AQP1, AQP4,and AQP8 in lung, AQP1, AQP8 and Na~+-K~+-ATPase in kidney;the self-recovery group rats Aquaporins protein content reduced than in the model group: AQP8 and Na~+-K~+-ATPase in liver, Na~+-K~+-ATPase in lung, AQP4 in kidney.After treated by Pingwei Power and alisma, Pingwei Power and alisma group rats Aquaporins protein content increased than in the self-recovery group:AQP4 and Na~+-K~+-ATPase in liver, AQP1, AQP4 and Na~+-K~+-ATPase in lung, AQP1, AQP4 and AQP8 in kidney;Pingwei Power and alisma group rats Aquaporins protein content reduced than in the self-recovery group:AQP1 and AQP8 in liver, AQP8 in lung, Na~+-K~+-ATPase in kidney.3 Distribution of proteins in liver, lung and kidney of rats:After animal model building, model group rats Aquaporins protein expression up-regulated than in the blank group:AQP1, AQP4 and AQP8 in liver, AQP1, AQP4 and AQP8 in lung, AQP1, AQP4 and AQP8 in kidney.After the intervention by Pingwei Power, Pingwei Power group rats Aquaporins protein expression up-regulated than in the self-recovery group:AQP8 in liver; Pingwei Power group rats Aquaporins protein expression down-regulated than in the self-recovery group:AQP1 and AQP4 in liver, AQP1, AQP4 and AQP8 in lung, AQP 1, AQP4 and AQP8 in kidney.After untreated by the self-recovery, the self-recovery group rats Aquaporins protein expression up-regulated than in the model group:AQP1 and AQP8 in liver, AQP8 in lung;the self-recovery group rats Aquaporins protein expression down-regulated than in the model group:AQP4 in liver, AQP1 and AQP4 in lung, AQP1, AQP4 and AQP8 in kidney.After treated by Pingwei Power and alisma, Pingwei Power and alisma group rats Aquaporins protein expression up-regulated than in the self-recovery group:AQP4 in liver,AQP1 in kidney;Pingwei Power and alisma group rats Aquaporins protein expression down-regulated than in the self-recovery group:AQP1 and AQP8 in liver, AQP1, AQP4 and AQP8 in lung, AQP4 and AQP8 in kidney.Conclusion:Syndrome of dampness in middle Jiao model not only can affect energy metabolism in the body but also can affect content and distribution of protein (AQP1, AQP4 and AQP8)in liver,lung and kidney,and it could be Syndrome of dampness in middle Jiao model leads to one of the mechanism of water metabolic imbalances;Pingwei Power can coordinate with the activity of Na~+-K~+-ATPase and content and distribution of protein (AQP1, AQP4 and AQP8)in liver.lung and kidney,involved in regulating energy metabolism and distribution of water metabolism, and it could be Pingwei Power one of the mechanism of drying wet of the spleen.