Regulation Of Lyplal1 Gene In Diet Induced Obesity Mice And Adipocytes Differentiation

Obesity, especially in west development countries, is an expanding health epidemics in the world. Besides harmful to health, obesity will lead a lot of social problems such as disemployment and discrimination. Recent years, Genome-wide association study (GWAS) about obesity has identified 52 gene variants that are significantly associated with obesity, these gene variants were considered to play key roles in the development and distribution of adipose tissues. Among these gene variants,4 SNPs (single-nucleotide polymorphisms near Lyplall (lysophospholipase-like 1) gene are identified and associated with obesity, especially in female.Lyplall gene encode a lysophospholipase-like 1 protein, a 26KDa cytosol protein that belongs to Lysophospholipase family or α/β hydrolases superfamily and homologous to human acyl protein thioesterase APt1 and APT2. However, the study performed by Burger shows that LYPLAL1 exhibits neither phospholipase nor triacylglycerol lipase activity. However, the exact biological function of this gene in obesity and during adipose tissue development remains uncharacterized.The purpose of this study is to uncover the role of Lyplall in obesity and distribution of adipose tissue. We first investigated the expression level of Lyplall in murine tissues and whether there is a sensual specificity of Lyplall expression. Then we focused on the change of Lyplall expression in high fat diet induced obese mouse. Besides, to study the regulation of Lyplall during the process of preadipocytes develop into mature adipocytes,3T3-L1 preadipocytes were induced by DMI and differentiated into mature adipocytes. What’s more, retroviral and lentiviral expression system were introduced to overexpress or knockdown Lyplall in preadipocytes then cells were induced into mature adipocytes. So we check that whether regulation of Lyplall disturb differentiation of adipocytes.Our results showed that Lyplall expression has a tissue specificity in normal mouse, in liver, epididymal white fat, skeleton muscle and kidney, Lyplall expression has a higher level than in small intestine, lung, adrenal gland, spleen. What’s more, there is a sex-specific expression profile in white adipose tissues (WAT) of mouse, in peri-uterine fat in female has higher expression compare in epididymal fat in male. In high fat diet induced obese mouse, we found murine Lyplall gene showed significant reduced in the epididymal and retroperitoneal fats depot. In vitro study showed Lyplall expression has a tendency of up-regulation during adipogenesis and it was enriched in mature adipocytes. However, based on retrovirus and lentivirus expression systems, overexpression and knockdown of Lyplall did not significantly disrupt adipocyte differentiation and triacylglycerol accumulation. All in all, our data determined a tissue-specific and sex-specific expression pattern of Lyplall in normal mouse, and a specific reduction of expression in diet induced obese mouse; however Lyplall was proved to be dispensable in adipocyte differentiation.