| Objective: It is to observe the changes of ERK5 expression in the neurons of spinal cord injury after spinal cord injury in rats,to explore the relationship between the expression of ERK5 and the neurons in the spinal cord injury,and to provide a new role in the treatment of spinal cord injury by using signal transduction pathway The experimental basis and research ideas.Methods: Sixty adult SD rats were randomly divided into three groups: experimental group(spinal cord injury model),sham operation group(open lamina,no spinal cord injury model)and control group(untreated).The model of rat thoracic spinal cord injury was established by modified Allen’s method.According to the rat brain stereotaxic map,the brain stereotaxic device was used to locate the rats before 48 hours.The unilateral lateral ventricle was injected with cholera toxin subunit B combined with horseradish peroxidase(CB-HRP)3μl.The BBB scores of the experimental rats were evaluated at1 day,3 days,7 days,14 days and 28 days after operation,and then the motor function of the hind limbs was evaluated.The expression of ERK5 was detected by immunofluorescence technique at the same time point.The expression of ERK5 was detected by immunofluorescence technique and the expression of ERK5.The both difference were analyzed at different time points by image analysis software,and then analyzed with SPSS19.0 statistical software.Results: 1、BBB score: experimental group of rats after spinal cord injuring modeling,leads to the hind limbs having serious motor dysfunction,lower extremity paralysis without self-activity,and BBB score is zero points.Formerly 3d is the most serious,BBB score of about 0-2 points,but with the extension of time,7-14 d recovery is more obvious.Therefore,the hind limb athletic ability in varying degrees of recovery,28 d or so is stabilized,BBB score of about 16-20 points.Sham operation group in the first 3d or so slightly lower than normal,about 19 points.The pain of its back of the trauma caused mouse activitylimited.However,the control group has no obvious change in the behavioral scores before and after,and the score was significantly higher than that in the experimental group.The sham operation group and the control group compared with the experimental group,BBB score was statistically significant(P<0.05);2、In the spinal cord tissue,the expression level of CSF-CNs/p-ERK5 positive cells began to increase at 1 day after spinal cord injury,and increased at about 7 days,and then began to decrease slowly.The expression level of CSF-CNs/p-ERK5 positive cells was relatively stable in the sham operation group and the control group.The expression of p-ERK5 positive cells was stable in the experimental group at different time points.Compared with the control group and sham operation Group,the difference was statistically significant(P <0.05).There was no significant difference between the sham operation group and the control group(P>0.05).There was no significant difference in the expression of CSF-CNs and p-ERK5 between the sham operation group and the control group.In the experimental group,CSF-CNs and p-ERK5double-labeled positive cells began to increase after 3 days,and the co-expression of CSF-CNs and p-ERK5 reached the maximum at about 14 days.The expression of CSF-CNs and p-ERK5 double-labeled positive cells was significantly lower than the control group and the sham operation group at 3 days,7 days,14 days and 28 days after the injury.The difference was statistically significant(P<0.05).There was no significant difference between the control group and the sham operation group(P>0.05).Conclusion: Intraventricular injection of CB-HRP can be well labeled CSF-CNs and the location is relatively fixed;the expression of CSF-CNs,p-ERK5 and CSF-CNs / p-ERK5 increased significantly with the time of spinal cord injury in rats.It indicates that the acute spinal cord injury may activate the ERK5 signaling pathway on CSF-CNs,to participate in the differentiation and protection of subsequent neurons,but the specific mechanism remains to be further studied and explored. |
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