Inhibiting Effects Of Hypoxia On The Treatment Of Naloxone And The Respiratory Rhythm Discharge Of Neonatal Rats

BackgroundBrain injury is a common disease in Department of Neurosurgery,due to its death rate,plant survival rate or mutilation rate is very high,often give the family greatly and even a devastating blow,while giving the society bring great burden.Brainstem injuries include spontaneous brainstem hemorrhage,brainstem infarction,and brain stem injury caused by progressive changes in brain injury and various types of cerebral hemorrhage.Research shows that the brain damage after the release of a large number of endogenous opioid peptides,cerebrospinal fluid of the patients with opioid class content increased obviously,naloxone(non-specific opioid receptor antagonist),can antagonize the effects of the substances.In brain injury patients,often with hypoxemia,resulting in adverse outcomes is an important factor in the process of treatment,patients with brainstem injury complicated with hypoxemia,found that patients with hypoxia treatment effect of naloxone on brain stem injury was significantly lower than that of brain stem injury have no obvious hypoxemia in patients.The brain stem is the location of the medullary respiratory center,which plays an important role in basic life activities such as breathing and heartbeat,so the research on the effect of naloxone and oxygen concentration in the brain stem disease,and even the use of animal brain slices experimental model of naloxone and oxygen concentration on respiratory function is very meaningful.According to this,we designed and completed the experimental study.ObjectiveThrough the observation and analysis of therapeutic effect of naloxone on patients with brain injury in neonatal rats,use of brainstem slice to investigate the effect ofnaloxone on neonatal rat medullary respiratory center in normoxia and hypoxia conditions,scientific and reasonable use of naloxone in the treatment of brain injury after respiratory rhythm abnormalities and provide experimental evidence.MethodsTo prepare neonatal rat medulla in vitro,and to record the basic rhythm of respiratory discharge with the adsorption electrode,and to observe the effect of naloxone on the basic rhythmic respiration.1 Clinical study: Patients with brain stem injury clinical study,group study of the treatment period(according to the GCS score after admission D1,D2,D3,D7,D11,D15,d30)changes,mortality,consciousness recovery time,brainstem function after 6 months of follow-up to the classification(GOS),the assessment of quality of life in rehabilitation period.To determine the efficacy of naloxone treatment in each group.2 Animal experiments: For verification regulation of naloxone on mNRF respiratory neurons of RRDA,different concentrations of naloxone and Propofol of prepared in vitro study specimens were perfused brain slices.To prepare neonatal rat medulla in vitro,and to record the basic rhythm of respiratory discharge with the adsorption electrode,and to observe the effect of naloxone on the basic rhythmic respiration.Results1 Clinical research Brain injury in patients with hypoxemia on disease improvement without significant deterioration;brainstem injury in patients with early use of naloxone on improvement of disease have significant positive effects;although the brain stem injury in patients with hypoxemia on disease improvement without significant deterioration,but the therapeutic effect of naloxone on hypoxemia of patients with poor in hypoxia using naloxone patients.2 Animal experiments Naloxone increased the excitability of inspiratory neurons in mNRF area,and Propofol inhibited the excitability of inspiratory neurons.20 mol / L naloxone can directly excite medullary slices of RRDA,a minimum concentration and decreases with the increase of concentration of excited enhancement,Propofol canantagonize the inhibition of RRDA slices,the effects are competitive to stop and replace Propofol with the receptor binding and implementation.ConclusionsThe oxygen concentration decreased slightly only mild effects of naloxone on brainstem slices of basic respiratory rhythm discharge excited function,moderate and severe oxygen concentration decreased when naloxone decreased excitatory effects on the brain slices of basic respiratory rhythm discharge at the oxygen concentration in a concentration dependent manner significantly decreased.