Analysis Of The Non-coding RNAs And Target Genes In The Interaction Of Differentially Expressed MiRNA In Osteogenesis Imperfecta

Circular RNA（circularRNA,circRNA）is widely exists in various kinds of biological cells different from traditional linear new form of RNA,enriched with strong annular structure in the cytoplasm,a large number of exists in eukaryotic transcription in the group.Most of the circularRNAs is made up of exon,good species conservative and tissue specificity,is not easy to be nuclease cracking.This makes circularRNA in clinical diagnostic markers as a new distributed application has obvious advantages.In terms of function,as a kind of new type circular RNA ceRNA modulator,circRNAs as a function of RNA sponge,competitive inhibition of the expression of microRNAs,blocking the miRNA target expression inhibition to its,so as to regulate the expression of other RNA.The researchers found that there are 11 kinds of miRNAs（miR-21、miR-26a、miR-29a、miR-29b、miR-30e、miR-34c、miR-133a、 miR-145、 miR-210、 miR-489 与 miR-1297）differentially expressed in osteogenesis imperfecta（P < 0.05）.The purpose of this study is to analyze the circRNAs,lncRNAs and target genes interacting with these differentially expressed miRNA by bioinformatics technology.Objective: CircRNAs,lncRNAs,and target genes are predicted to interact with known osteogenesis deficient serum miRNAs,and their association with signaling pathways is analyzed.Based on bioinformatics prediction,a preliminary analysis of the predicted circRNA,the expression of target gene miRNA in bone and bone tissues.Methods:To predict the differential expression of eleven kinds of miRNA in the serum of osteogenesis imperfecta showed the corresponding circRNAs,lncRNAs and target gene by starbase and miRWalk database,meanwhile,bioinformatics analysis of miRNAs,circRNAs,lncRNAs and target genes by DAVID and Cytoscape software.Trizol was used to extract the discarded bone tissue from 8 patients with osteogenesis osteogenesis,and 8 patients with congenital dislocation of the hip were used as controls.Through the method of RT-QPCR to study the expression of has_circ₀00713,has_circ₀01439 and has_circ₀01499 three circRNA,other eight target genes NTN4,PPP2 CA,SRGAP1,YES1,FAT1,KIAA1586,LMNB2,RFC1.The specific primers were screened by real-time quantitative PCR.Results:Eleven miRNA related circRNAs,lncRNAs prediction by starbase.The total number of circRNAs with its interaction is 222 and the non overlapping circRNAs is 141.The total number of lncRNAs with its interaction is 359 and the non overlapping lncRNAs is 215.DAVID,mi RNAs,circRNAs,Cytoscape software of lncRNAs,the target gene by bioinformatics analysis of miR-29a/miR-29b/miR-133 a and KIAA1586_hsa_circ₀01439,PPP2 CA gene interactions exist,and included in the TGF-beta,Tight and junction in WNT signal pathway.MiR-133a/miR-145 interacts with lincRNA,RP11-156E6.1,FAT1_hsa_circ₀00713,LMNB2_hsa_circ₀01499,and YES1,and may be related to the Tight junction signaling pathway.Mi R-26a/miR-145 and LncRNA,HNRNPU-AS1,MALAT1,AL589743.1 molecular RP11-478C19.2 four LncRNA,RFC1_hsa_circ₀01649,circRNA,NTN4 interacts with the SRGAP1 gene,which may be related to the Axon guidance signaling pathway.On the basis of the above bioinformatics prediction,RT-QPCR results showed that the predicted three circRNA showed no significant difference in the experimental bone tissue.LMNB2,NTN4,RFC1 and SRGAP1 genes were differentially expressed in experimental bone tissue.Conclusions: Forecast analysis and construction of the relationship between circRNAs/lncRNAs and target genes of 11 known reported oi serum differential expression of miRNA and interaction,and its localization in the specific signaling pathway in.It lays a foundation for further study of their interaction.