Oscillating Field Stimulation On Expression Of β-catenin Protein And Neurons Regeneration In Rat With Spinal Cord Injury

Objectives: To explore the influence of oscillating field stimulation on expression of β-catenin protein and neurons regeneration in rat with spinal cord injury.Methods: Sixty SD rats with spinal cord injury induced by Allen′s method was randomly divided into control group and experimental group,thirty rats in each group.All rats in the experimental group were implanted oscillating electrical field as intervention,while the control group only reveived oscillating electric field stimulator but not applied stimulus.At3,7,14 d after experiment,Basso-Beattle-Bresnahan(BBB)Rating Scale was used to analyze the recovery of motor function.Western blot,immunofluorescence and immunohistochemical staining were used to observe the expression of β-catenin protein and neurons regeneration of SD rats.The statistical analysis was performed between 2 groups.Results: BBB scores showed no significant difference at 3,7 d in 2 groups(P>0.05),while BBB scores presented a significant difference at 14 d(P<0.05).Western blot and immunohistochemistry results showed that the expression of β-catenin protein was higher than the control group at each time point(P<0.05),and the expression of β-catenin protein gradually decreased with the extension of the time in 2 groups.Neurons expressed an increasing trend from 3 d to 14 d,while the number of neuron in the experimental group expressed better than that in the control group,and cell morphology were more regular.Conclusion: Oscillating field stimulation can improve the expression of β-catenin protein and neurons regeneration in the injured spinal cord,which would contribute to neurological improvement after spinal cord injury.