The Effect Of Adipose-Derived Stem Cell Sheet Combining Tricalcium Phosphate/Collagen Fiber Scaffold To Improve Cell Osteogenesis

Objective:Although cell-based substitute material transplantation has been considered an optimal choice for the bone regeneration,it is far from ideal because existing scaffold material and suitable seeding cell sources should be consummated.In the present study,we presented a novel compound composited with adipose-derived stem cell(ADSC)sheet and beta-tricalcium phosphate/collagen fiber(β-TCP/CF)scaffold,examined its osteogenic activity in vitro.Methods:We treated the β-TCP/CF scaffold wrapped around by a monolayer ADSC sheet and cultured in osteoinducing mediumas the experimental group(Group A),while the β-TCP/CF scaffold combined with ADSCs(Group B)and a mere monolayer ADSC sheet(Group C)were treated as controls.The proliferation of ADSCs on the novel composited scaffold was determined by MTTassay.Osteogenic differentiation was confirmed by activity of alkaline phosphatase(ALP)andsecretion level of osteocalcin(OC),and the deposition of calcium was detectedby the staining of Alizarin red S.Results:The results of MTT show that cell proliferation on theβ-TCP/CFscaffold was similar to conventional culture plates.When compared withβ-TCP/CF scaffold-ADSCs combination,β-TCP/CF scaffold-ADSC-sheet combination had a significant stronger ALP activity on Days 3,5,7,10,and 14,and significant higher OC level on Days 3,5,and 7(p<0.05).When compared withmere monolayer ADSC sheet,β-TCP/CF scaffold-ADSC-sheet combination had a significant stronger ALP activity on Days 5,7,10,12,and 14,and significant higher OC level on Days 5,7,10,and 12(p<0.05).Conclusions:We concluded that the compound composited with ADSC cell sheets andβ-TCP/CF scaffold might be a promising candidate for bone-engineered.