Studies On The Constituents From Yao Medicine Tetrastigma Planicaul

Objective:Study on the chemical constituents from Tetrastigma planicaul（Hook.）Gagnep.This work can give a scientific basis for ex-posing the antitumor activity extracts from the plant.Methods:T.planicaul was extracted five times by 80%ethanol,and the extract was separated into five parts according to the polarity,including petroleum ether part,ethyl acetate part,n-butanol part and wa-ter part.The constituents were isolated by silicagel,macroporous resin adsorption,polyamide,Sephadex LH-20 chromatography,recrystallization,et al.The structure were identified by spectral analysis(MS,¹H-NMR,¹³C-NMR et al)and comparing with the published data.The active ingr-edient protocatechuic acid of T.planicaul was qualitatively identifiedby TLC,and quantitative dermined by HPLC.Result:Twenty-four compounds were isolated and identified as coelarthenol（1）,ethyl 3β-hydroxy-5-cholen-26-oate（2）,taraxerone（3）,β-sitosterol（4）,daucosterol（5）,protocatechuic acid（6）,dennettine（7）,ethyl gallate（8）,tetradecanoic acid（9）,1-dodecanol（10）,glyceryl pal--mitate（11）,3-β（stearyolxy）olean-12-ene（12）,palmitic acid（13）,salicy-lic acid（14）,β-sitosteryl oleate（15）,vanillic acid（16）,stigmasterol（17）,12-methyltridecan-1-ol（18）,（24s）-3β-hydroxy-5α-stigmastan-6-one（19）,isoscopletin（20）,estigmast-4-en-6β-ol-3-one（21）,stigmast-4,2,2-dien-3-one（22）,D-glucose（23）,D-mannitol（24）.Content of protocat--echuic acid of T.planule had a good linearity in the range of 0.0292⁰.584μg（r=0.9998）.The result of methodology validation meets the re-quirement.Conclusion:Compound 1³,7,9,10,12,15,18²2 are iso-lated from Tetrastigma BI.for the first time.1³,7,9,10,12,15,18²4 are obtained from this plant for the first time.The method can be used for the estimation of quality of T.planule.