| ObjectiveTo explore the effects of supplementation of n-3 polyunsaturated fatty acids(n-3 PUFA)on fine particulate matter(PM2.5)exposed type 2 diabetes mellitus(T2DM)rats.MethodsTotal of 70 at four weeks of age male SD rats were randomly divided into normal control group(NC)and diabetic molding group(MD)according to the body weight,fed with normal diet and high fat diet respectively.Six weeks later,the rats in the MD group were injected with streptozotocin(STZ)intraperitoneally to establish T2 DM animal models.A week after STZ injection,the successfully established T2 DM rats were randomly divided into 6 groups including T2 DM control group(T2DM group),PM2.5 group,corn oil control group(CO group)and three fish oil(FO)feeding groups(given three dosages of fish oil,named as L-FO,M-FO and H-FO group).NC group and T2 DM group were inoculated with saline.The other groups were inoculated with suspensions of PM2.5 at 5 mg/kg concentration particulate via trachea once a day for a week.The rats in FO groups were administered with fish oil containing n-3 PUFA at dosages of 75,150,300 mg/kg by gavage for L-FO,M-FO,and H-FO groups respectively for continuous 4 weeks.At the same period,rats in CO group were given equal volume of corn oil.At the end of experiment,the rats were killed under aneasthesia,the blood was drown for measurements of lipid profile,glucose,uric acid(UA),C-reactive protein(CRP)and interleukin-6(IL-6).The morphology of hepatic tissue was checked under optical microscope.The data from all groups were processed,t-test was used to analyze the difference between groups and multiple-group comparison was carried out using one-way analysis of variance(ANOVA).The significant level was set at α=0.05.Results1.Compared with the NC group,the rats in T2 DM control group showed significant decreased body weight(335.8±88.8 vs.509.6±21.9),but increased consumption of food and water and urinary discharge(36.9±8.8 vs.20.0±3.1,203.2±35.2 vs.27.7±4.6,179.5±19.5 vs.18.7±1.5)(P<0.05).Supplementation of n-3 PUFA did not significantly change the body weight,food and water consumption,urinary discharge in the PM2.5 exposed T2 DM rats(P>0.05).2.Serum glucose level(19.63±4.19),total cholesterol(1.85±0.52),triglycerol(TG)(1.42±0.69)and uric acid(SUA)(143.57±10.44)were significantly higher in the T2 DM control group rats than those in NC group(5.29±1.20,1.21±0.16,0.55±0.12,103.38±6.91)(P<0.05).PM2.5 exposure significantly increased serum TG(1.42±0.69)and SUA(143.57±10.44)levels in the T2 DM rats(P<0.05).The administration of FO with low,medium and high levels of n-3 PUFA made decline of SUA in the T2 DM rats exposed to PM2.5(129.50±21.44,123.43±26.79 and 97.83±9.62 vs.180.14±29.07)(P<0.05),but not significant changes in lipid profile and glucose levels.3.Serum levels of CRP and IL-6: the contents of CRP(26.91±4.17)and IL-6(120.22±51.60)were significantly higher in the rats of T2 DM control group than those in the NC group(14.84±1.17,57.81±5.94)(P<0.05).The T2 DM rats exposed to PM2.5 showed significantly increased serum IL-6 level(197.48±46.42),while supplementation of n-3 PUFA significantly decreased IL-6 level in the PM2.5 exposed T2 DM rats(84.29±24.17,91.06±29.30 and 88.82±23.28 vs.162.46±68.10)(P<0.05).Conclusions1.PM2.5 exposure could increase serum levels of TG,SUA and IL-6 in T2 DM rats.2.n-3 PUFA could markedly reduce the serum levels of SUA and IL-6 in T2 DM rats exposed to PM2.5. |
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