| bjective: To investigate whether androgen through AKT3,PIK3 CA,CALM,CAV1 regulate the eNOS expression in corpus cavernosum tissues of rats,thus affecting erectile function.Methods: 8-week-old male SD rats were randomly divided into 6 groups,4 weeks in the control group(group A),6weeks in the control group(group B),4 weeks castration group(group C),6Weeks in potential group(group D),4 weeks castration + testosterone replacement(group E),6 weeks castration + testosterone replacement(group F).C,D,E,F group,removal of both testis and epididymis,One day later,group E,F subcutaneously injected with testosterone propionate 3mg / Kg every other day,the other groups with isodose oil instead.(Group A,C,E)after 4 weeks,(group B,D,F)were detected within each group of rats maximum intracavernous pressure(ICPmax)/ mean carotid arterial pressure(MAP)6weeks,serum testosterone(T),eNOS,P-eNOS,AKT3,PIK3 CA,CALM,CAV1 expression in the penis of rats in each group were measured by Western blot Western blotting and immunohistochemistry.Results: The body weight(Group A:281.9±6.4g,Group B:284.2±3.5g,Group C:280.9±3.8g,Group D:284.9±2.8g,Group E:282.5±3.2g,Group F:283.7±2.3g)and mean arterial pressure strength(Group A:112.2±2.5mmHg,Group B:113.1±3.6 mmHg,Group C:108.2±3.3mmHg,Group D:114.5±8.9 mmHg,Group E:110.9±4.3mmHg,Group F:116.1±2.4mmHg)of rats was no significant difference.Serum T values:The expression of castration group(group C:1.08±0.23nmol/L,group D:0.85±0.20nmol/L),compared with control group(group A:18.27±1.15nmol/L,group B:18.43±0.91nmol/L)and castration + testosterone replacement group(group E:17.86±0.47nmol/L,group F:17.91±0.35nmol/L)decreased significantly(P<0.01).Compared to castration + testosterone replacement group(group E,F)and control group(group A,B),the expression have no significant difference.And 6 weeks castration group(group D)decreased significantly than4 weeks castration group(group C)(P <0.05).By measuring ICPmax/MAP in each group at 3V,5V voltage stimulation,The expression of castration group(group C:0.28±0.05,0.38±0.04、group D:0.19±0.03,0.24±0.04),compared with control group(group A:0.71±0.06,0.88±0.03、group B:0.73±0.05,0.86±0.03)and castration + testosterone replacement group(group E:0.62±0.07,0.81±0.05 、group F:0.64±0.04,0.82±0.02)decreased significantly(P <0.01).Compared to castration + testosterone replacement group(group E,F)and control group(groupA,B),the expression have no significant difference.And 6 weeks castration group(group D)decreased significantly than 4 weeks castration group(group C)(P <0.05).Immunohistochemistry: eNOS,P-eNOS is mainly expressed in vascular endothelial cell membrane and cavernous endovascular;AKT3,PIK3 CA,CALM,CAV1 mainly expressed in vascular endothelial cell cytoplasm and membrane,a few expressed in smooth muscle cells.Western blotWestern blot analysis showed eNOS,P-eNOS,AKT3,PIK3 CA,CALM,CAV1:The expression of castration group(group C:0.24±0.04,0.26±0.03,0.44±0.05,0.34±0.06,0.31±0.04,0.14±0.02,group D:0.11±0.02,0.25±0.03,0.27±0.03,0.22±0.04,0.11±0.02,0.14±0.03),compared with control group(group A:0.82±0.05,0.72±0.04,0.98±0.07,0.96±0.09,0.68±0.07,0.69±0.08 、 group B:0.92±0.09,0.72±0.06,0.94±0.08,0.89±0.07,0.74±0.06,0.69±0.08)and castration +testosterone replacement group(group E:0.87±0.08,0.76±0.07,0.94±0.09,0.86±0.08,0.78±0.07,0.79±0.08 、 group F:0.96±0.09,0.82±0.08,0.95±0.09,0.93±0.07,0.84±0.06,0.75±0.07),decreased significantly(P <0.01).Compared to castration + testosterone replacement group(group E,F)and control group(groupA,B),the expression have no significant difference.And 6 weeks castration group(group D)decreased significantly than 4 weeks castration group(group C)(P <0.05),compared to 6 weeks castration + testosterone replacement(group F)and 4 weeks castration + testosterone replacement group(group E)hane no significant difference.Conclusion: androgens can increase the expression of AKT3,PIK3 CA,CALM,CAV1 protein molecules,after eNOS phosphorylation of eNOS activation,to improve erectile function. |
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