Material Basic Study About Liver Injury Of Dioscorea Bulbifera L.

Objective:Based on the use of tools and liver injury experiment to study the constituent of Dioscorea bulbifera L.and find the parent compound with liver injury activity.Methods:The Dioscorea bulbifera L.was refluxing and extraction with 75%ethanol,and extraction with petroleum ether,ethyl acetate and n-butanol.Take the appropriate amount of the extraction site for liver injury experiments to determine the main liver injury sites.The major liver injury sites were isolated and purified by various chromatographic elution separation methods,and nuclear magnetic resonance spectroscopy of the monomer were isolated structure determination.The experiment of screening of antitumor activity o and partial liver injury was carried out by using some monomeric compounds.The HPLC fingerprints of different batches of Dioscorea bulbifera L.were compared by high performance liquid chromatography.Results: The liver injury of mice in each extraction site showed that liver injury was the most serious in the extractive fraction of ethyl acetate compared with the blank group,followed by n-butanol extraction,and the liver injury was the least in the petroleum ether extract.44 monomer compounds were isolated from the ethyl acetate fraction of Dioscorea bulbifera L.,which were identified as diosbulbin E acetate(1),diosbulbin R(2),diosbulbin S(3),diosbulbin phenol Ⅰ(4),diosbulbin phenol Ⅱ(5),(±)1-hydroxyl-meso-hannokinol(6),8-epidiosbulbin E acetate(7),diosbulbin G(8),diosbulbin B(9),diosbulbin D(10),diosbulbin C(11),diosbulbin A(12),diosbulbin F(13),epicatechin(14),5,3 ’,4’-trihydroxy-3,7-dimethoxyflavone(15),catechins(16),5,7-dihydroxy-3,4’-dimethoxyflavone(17),3,5,7,4’-tetrahydroxyflavone(18),3,5,3’-trimethoxyquercetin(19),3,5,7,3’,4’-pentahydroxyflavone(20),2’,3-dihydroxy-4,5-dimethoxybibenzyl(21),2’3-dihydroxy-5-methoxybibenzyl(22),batatasin Ⅲ(23),tristin(24),2,7-dihydroxy-3,4-dimethoxyphenanthrene(25),3,7-dihydroxy-2,4-dimethoxy-9,10-dihydrophenanthrene(26),2,3,7-trihydroxy-4-methoxyphenanthrene(27),ephemeranyhoquinone(28),vomifoliol(29),teasperol(30),4,5-dihydroblumenol A(31),aurantiamide acetate(32),methyl eucomate(33),3,4-dihydroxybenzoic acid methyl ester(34),dihydroxybenzoic acid ethyl ester(35),P-hydroxybenzoic acid(36),3-hydroxy-1,7-bis-(4’,4’’-dihydroxyphenyl)-heptane(37),platyphyllenone(38),C-veratroylglycol(39),P-hydroxyphenylacetic acid methyl ester(40),3,4-dihydroxybenzoic acid(41),Z-6,7-ligustilide(42),N-methyl-2-pyrolidinone(43),daucosterol(44).The animal experiments of compounds 1,7,11,12,13 showed that compared with the blank group,the levels of hepatotoxicity were different in different compound groups,and there was a certain relationship with the dose.The HPLC fingerprint methods of Dioscorea bulbifera L.were established,and the principal components and cluster analysis of different batches of herbs were carried out by means of mathematical analysis.Conclusion: The main components of the liver injury of Dioscorea bulbifera L.are the ethyl acetate.44 compounds were isolated from the ethyl acetate fraction,among which compounds 1-6 are new compounds,and 17,18,20,21,23,25,26,28-36,38-40,42-44 were isolated from this plant for the first time.Compound 1,7,11,12,13 were subjected to mice liver injury test,the levels of hepatotoxicity were different in different compound groups,and there was a certain relationship with the dose.