Expression And Significance Of FAT4 And YAP1 In Epithelial Ovarian Carcinoma

Objective: Epithelial ovarian tumor is the most common ovarian tumors in the female reproductive system.When the patient once diagnosed,the numble of patients are more than 70% in advanced,resulting in poor clinical outcome.To threatened the women’s life and quality of life seriously.so the mechanism of tumor occurrence and development has done a lot of research,in which epithelial tissue Hippo pathway in recent years,a new found a signal pathway.The main physiological function of Hippo pathway is balance to between cell proliferation and apoptosis,and control the size of tissues,organs and the internal environment of the homeostasis.More and more studies have shown that the abnormal regulation of Hippo signaling pathway is closely related to the occurrence and the development of malignant tumors,leading to imbalance of proliferation and apoptosis of tissue cells,enhancement of invasion and migration ability and tumor formation.FAT4 and YAP1 are the upstream and the downstream molecules of Hippo signal pathway and participate in tumorigenesis and progression.Our experiment is aim to detect the expressions of FAT4 and YES-associated protein1(YAP1)in epithelial ovarian cancer through immunohistochemical method and the correlation of YAP1 and FAT4 in patients with ovarian epithelial carcinoma.so as to provide reference for ovarian cancer research.Methods:1).Study groups: Forty-five samples of epithelial ovarian cancer in patients undergone operation in our hospital from December 2014 to December 2015 were enrolled as experimental group(including 31 cases with serous ovarian cancer,14 cases mucinous cystademocarcinoma).And 25 cases of benign ovarian epithelial tumor tissue(including 18 cases ovaryserous cystadenoma and 7 cases mucinous cystadenoma)were selected as control.In accordance with MDACC,according to the organization will be statistical study of grading.2).Specimen collection: All ovarian tissues were obtained from postoperative.Ovarian tissues were immediately dissected and cutted into sizes of approximately 1 * 1 * 1cm,to-80 ℃ refrigerator frozen reserve,embedded in paraffin later.3).Measurement indexes: The expression of FAT4 and YAP1 in the ovarian tissues were detected by immunohistochemical techniques.The correlateon between the expression of FAT4,YAP1 in epithelial ovarian cancer and histopathological grading.The correlation between FAT4 and YAP1 in ovarian tissues.4).Statistical analyses:SPSS 16.0 was applied for all statistical analysis.Comparisons among groups were analyzed by chi-square test,and the correlation between FAT4 and YAP1 was analyzed by Spearman rank correlation analysis.Take a = 0.05 as the test level,P <0.05 was considered statistically significant.Results:1)The positive expression rates of FAT4 and YAP1 in ovarian cancer were84.4% and 75.5%,but in ovarian epithelial benign tumors the positive expression rates of FAT4 and YAP1 were 44% and 40%,respectively,the different was statistically significant(P<0.05).In cancer group,the positive expression rates of YAP1 and FAT4 protein increased gradually in patients with poorly differentianted,the difference was statistically significant(P<0.05).2)In ovarian cancer tissues,the relationship of FAT4 and YAP1 was positive correlated with Spearman correlation coefficient(r=0.565,P<0.001)Conlusions:The expression of FAT4 and YAP1 protein in epithelial ovarian cancer was significantly higher than that in ovarian cancer.Through this study,we believe that FAT4 and YAP1 can be used as a biomarker for the diagnosis of epithelial ovarian cancer,which can be used as a reference index for the classification of epithelial ovarian cancer.Can provide a new idea for targeted therapy of ovarian cancer.