Hyperthermia Effect Of Au Nanoparticle Cage-Quantum Dots-AFP Combination Probe Targeting On Liver Cancer Stem Cells

Object: By detecting the thermo-therapy sensitivity of water-soluble Au Nanoparticle Cage-Quantum dots-AFP combination probe targeting to HepG2 cells with different concentrations to explore the feasibility of thermotherapy in the treatment of cancer stem cells.Methods: Immunofluorescence was used to confirm that HepG2 cells can express AFP antigen,and then AFP was selected as target antigen. Silver cubic nanocrystalline and hypochlorous acid was used to prepare gold nanocages by replacement reaction method for photothermal therapy. Hydrothermal synthesis was performed to prepare CuInS2-ZnS quantum dots to conduct cell fluorescence staining. Coupling gold nanocages,quantum dot sum and anti-AFP into Gold Nanometer Cage-Quantum Dot-Anti-AFP composite probe. After confirming that Hep-G2 stem cells can express Nanog antigen via immunohistochemical, HepG2 stem cells with different concentrations wereselected to incubate with compound probe at the concentration of 20%,and then they were exposed to laser (wavelength: 808 nm and power density: 1.5 W / cm2). The apoptosis of HepG2 cells was detected by flow cytometry. The survival rate of HepG2 cells was detected by fluorescent terminators.Results: Immunohistochemical staining showed that a large number of cells HepG2 had positive staining of cytoplasm after combing AFP antigens,and a small number of HepG2 and Nanog antigens had nuclei positive staining after combing Nanog antigens and algebraic positive staining decreased over time (t=8.916 , P<0.001 ). HepG2 cells in different generations were co-cultured with compound probe at the concentration of 20% and then they were exposed to laser. The percentage of apoptotic cells increased when the concentrations of Hep-G2 decreased and the lighting time prolonged (t=5.031,P=0.001) . Observation with a fluorescence terminator staining microscope showed: under the same conditions,the more stem cells,the less ones showed yellow.Conclusion: (1) The Au Nanoparticle Cage-Quantum dots-AFP combination probe targeting can combine to HepG2 cell line and be traced. (2) Nanog immunohistochemical staining showed that HepG2 contained a small number of nucleus expressing Nanog-positive stem cells,and the number of stem cells over time.(3)The results of flow cytometry and fluorescent terminators showed that tumor stem cells were more resistant to heat than normal tumor cells.