IL-17 And IL-35 Serum Levels In Psoriasis Vulgaris Patients And Their Correlation With PASI Score

ObjectiveTo detecte the expression levels of IL-17 and IL-35 in the serum of patients with psoriasis vulgaris,and to analyze their changes in different stages and their correlation with PASI score,to further explore their role in the pathogenesis of psoriasis,providing the basis for the future treatment of psoriasis vulgaris.Methods1.Research object: 29 patients with psoriasis vulgaris were observed in our hospital from February to November 2016.Selection criteria:(1)Such patients have obvious psoriasis-like lesions.(2)Excluded dermatomyositis,pemphigus,lupus erythematosus,chronic urticaria,malignant tumors and other autoimmune diseases and its associated with other systemic diseases on the basis of "diagnostic criteria for psoriatic clinical dermatology".(3)They haven’t treated with Immunosuppressants,Accutane,Corticosteroids hormone and other autoimmune drugs within 3 months.These patients were divided into two groups,including the progressive group(n=16),and the stationary group(n=13).The PASI score was recorded by the same staff for psoriasis patients.The healthy control group(29 cases)were all from the health examination center of our hospital,they had no autoimmune disease and family history,and in gender and age there was no significant difference between them with t-test.2.Methods: The levels of IL-17 and IL-35 in the serum of psoriasis patients and healthy controls were detected by double antibody sandwich enzyme-linked immunosorbent assay(ELISA),and then the staff records the results.3.Statistics: The data were analyzed by SPSS 21.0 software.The quantitative data are expressed as mean±standard deviation(x ±s).The mean of the measurement data between two groups compared with t-test,while multiple groups compared with single factor variance analysis(variance using LSD method,the heterogeneity of variance using DunnettT3 method),categorical variables expressed as a percentage were compared with chi square test.Pearson correlation analysis was used to analyze the correlation of two continuous random variables.P < 0.05 represents the difference was statistically significant.Results1.The expression level of IL-17 in the serum patients with psoriasis vulgaris is higher than in the healthy control group,the difference was statistically significant(P < 0.05),and the expression level of IL-17 in the serum of patients with progressive stage is higher than in stationary stage,the difference was statistically significant(P < 0.05).2.The expression level of IL-35 in the serum patients with psoriasis vulgaris was lower than of healthy control group,the difference was statistically significant(P < 0.05),and the expression level of IL-17 in the serum of patients with progressive stage is lower than in stationary stage,the difference was statistically significant(P < 0.05).3.The expression levels of IL-17 and IL-35 in serum of patients with psoriasis vulgaris were negatively correlated(P < 0.05).4.The expression level of IL-17 in the serum patients with psoriasis vulgaris was positively correlated with PASI score(P < 0.05).The expression level of IL-35 inthe serum patients with psoriasis vulgaris was negatively correlated with PASI score(P < 0.05).Conclusions1.IL-17 may act as a proinflammatory cytokine and contribute to the psoriasis vulgaris;IL-35 may as a protective factor involved in the pathogenesis of psoriasis vulgaris;and they may be related with the course of psoriasis vulgaris.2.The imbalance of IL-17 and IL-35 may be involved in the pathogenesis and overexpression of immune responses of psoriasis vulgaris.