The Function And Mechanism Related To MiRNA Of Breast Cancer As Demonstrated By Ring1 And YY1 Binding Protein Expression

Breast cancer is the most common cancer among woman and the second leading cause of cancer-related death in the world.However,the mechanism underlying of breast cancer still remains unknown.Ring 1 and YY1 binding protein(RYBP)exerts in polycomb repressive complex 1(PRC1)and mediates the H2 A ubiquitination,so plays an important role in gene silencing and cellular differentiation.At present,abnormal expression of RYBP is found in many human cancers.Here,the study was designed to investigate the role of RYBP and its potential mechanisms related to miRNA on breast cancer(BC).Human BC tissues results revealed a statistically significant enforce in human breast tumor compared with peritumoral tissues.Knockdown of RYBP in BC cells led to significant decrease in cell proliferation,migration,invasion and stenmess in vitro and vivo.Downregulation of endogenous RYBP induces sensitivity of anthracyclines in advanced BC cells.In addition,over-expressed RYBP in human epithelial mammary(HEM)cells occurred the opposite characteristics.In further research,human miRNA array results demonstrated multiple changes of miRNAs associated with RYBP,including miR-206.And miR-206 is negatively correlated with expression of RYBP.Down-regulation of miR-206 in cells with low level of RYBP was obviously correlated with EMT and re-expression of nucleolin and VEGF.Taken together,our results demonstrated that RYBP could decrease the level of miR-206 and contribute to aberrant cell malignant causing tumorigenesis.