Study On CGMP Selected Control Channel Of Retinal Photoreceptor Cells With Relationship Of Light Damage And Aging Defense Mechanisms Reaction

Purpose To study the relationship between the cGMP control channel of the retinal photoreceptor cells and the aging response time of different light times,and clarify the protective mechanism.Methods 661w cells were exposed to 4500 Lux visible light,we set three periods : 3 days,4 days and 5 days respectively.and also set three light periods per day for the corresponding time period(20minutes,30 minutes,60 minutes,1.5 hours,2 hours,3 hours),the rest of the time is in the incubator,without any other treatment.Cell activity and cell death rate was measured with Hoechst / PI(diphenylmethane / propidium iodide)staining,Calcium ion detection of calcium ion expression;The changes of GCAP1 and cGMP landmarkers in cGMP control channel were detected by Western Blot and fluorescence quantitative PCR respectively at protein level and m RNA level.Results1.Whether in the dark or light environment,661 w cells in 3 days of low mortality and little difference.but the mortality rate was significantly higher from the fourth day,the cell death rate in the light was higher than that in the dark,and the cell death rate increased gradually with the prolongation of the irradiation time.2.cGMP control channel regulates the relevant factors : GCAP1 and cGMP have specific expression in protein and m RNA levels.After three days of illumination,GCAP1 and cGMP were significantly expressed at 20 min and 1.5 h;After four days of illumination,GCAP1 and cGMP were significantly expressed at 20 minutes and 60 minutes.After five days of light exposure,GCAP1 and cGMP were significantly expressed only at 30 minutes and no significant expression at other times.Conclusions:the survival rate of 661w cells has been relevant to light and time;The changes in expression of GCAP1 and cGMP may be related to light-induced damage to cell metabolism and restoration.