Effect Of High Retention Enema With Chinese Medicine On ICC And Serum VIP Of Colonin STC Rats

Objective:To observe the effect of high retention enema with Tongfurunchang Decoction on theinterstitial cells of Cajal(ICC)and serum vasoactive intestinal peptide(VIP)inslowtransit constipation(STC)rats.To investigate the effect of high retention enema with Tongfu runchang Decoction and mechanism in the treatment of STC.Methods: 60 SD rats were randomly divided into the group A(control group),groupB(constipationmodel group),groupC(mosapride group),groupD(high retention enemawith low dose Chinese medicinegroup),groupE(retention enemawith moderate dose Chinese medicine group),group F(high retention enemawith high dose Chinese medicinegroup).During the modeling,the compound diphenoxylate wasadministrated by gavage in groupB,C,D,E andF.The normal saline was administrated by gavagein group A.After successful modeling,the rats wereadministrated by gavagewith normal salinein groupA and B.The rats in the other groups were treated with the corresponding drugs.The general conditions,number offaecesand fecalmoisture contents in rats within 24 h were observed before and after successful modeling as well as after the treatment.The pushing percentage of carbonicpowder,serum VIP concentration and ICC in the colon of SD rats were detected after the treatment.Results:(1)Compared with group B,the numberof feces ingroup C,D,E,F,p=0.000,p<0.05;Compared with groupC and D,the number of feces ingroup E,p=0.001,p<0.05;Compared with groupF,the number of feces ingroup C and D,p=0.001,p<0.05;Compared withgroupC,the number offeces ingroup D,p=0.930,p>0.05;Compared with groupE,the number of feces in groupF,p=0.784,p>0.05.(2)Compared with groupC,the fecal moisture content in group B,p=0.820,p>0.05;Compared with groupB,the fecal moisture content in group D,E and F,p=0.002,0.001 and 0.001,respectively,p<0.05;Compared with groupD,the fecal moisture contentin group E and F,p=0.721 and 0.752,p>0.05;Compared with groupE,the fecal moisture content in group F,p=0.960,p>0.05;(3)Compared with group B,the pushing percentage of carbonicpowderin group A,p=0.000,p<0.05;Compared with group B,the pushing percentage of carbonicpowderin group C,D,E and F,p=0.000,p<0.05;Compared with groupE,the pushing percentage of carbonicpowderin group C and D,p=0.000,p<0.05;Compared with groupF,the pushing percentage of carbonicpowderin group C and D,p=0.710,p>0.05;Compared with groupC,the pushing percentage of carbonicpowderin group D,p=0710,p>0.05;Compared with groupE,the pushing percentage of carbonicpowderin group F,P=0.964,p>0.05.(4)Compared with group B,theVIP concentration in group C,D,E and F,p=0.000,0.001,0.000 and 0.000,p<0.05;Compared with group C,theVIP concentration in group D,E and F,p=0.593,0.954 and 0.643;Compared with group D,the VIP concentration in groupE and F,p=0.642 and 0.933;Compared with group E,the VIP concentration in group F,p=0.694,p>0.05.(5)Compared with group B,the number of ICC in group C and D,p=0.311 and 0.106,p>0.05;Compared with group B,the number of ICC in group E and F,P=0.006 and 0.020,p<0.05.