Expression Of β-catenin,Wnt3a And Lgr5 In Different Cervical Tissues And Their Correlation

Objective: To detect the expression of β-catenin,Wnt3 a and Lgr5 in different cervical tissues,and analyze the correlation among the expression,to explore their relationship with the occurrence and development of cervical cancer.Methods: The expression of β-catenin,Wnt3 a and Lgr5 in different cervical tissues were detected by immunohistochemical method（SP method）,including 61 cases of cervical cancer tissue,30 cases of cervical intraepithelial neoplasia（12 cases of CINI,18 cases of CINII-III）,19 cases of normal cervical tissue.Results:1.Under normal conditions,the expression of β-catenin was continuous in the cell membrane,the pathological state,there is a loss of membrane expression,and a different level of abnormal expression in cytoplasmic/nuclear.The abnormal expression rate of β-catenin was 5.26%（1/19）in normal cervical tissue,33.33%（4/12）in CINI group,38.89%（7/18）in CINII-III group and 75.41% 61),comparison between 4 groups（χ₂=32.958,P <0.05）,with statistically significant.The abnormal expression rates of G1,G2 and G3 grade were 41.67%（5/12）,76.67%（23/30）,94.74%（18/19）in cervical cancer,It can be seen that it is prominent in G3 group expression,（χ₂=11.221,P <0.05）,with statistically significant.The abnormal expression rates in the shallow infiltration group and the deep infiltration group were 55.56%（10/18）and 83.72%（36/43）（χ₂=5.428,P <0.05）,with statistical significance.The abnormal expression may not relate to clinical stage,age,histological type and age（P >0.05）.2.The positive expression rate of Wnt3 a was 10.53%（2/19）in normal cervical tissue group,41.67%（5/12）in CINI group,55.56%（10/18）in CINII-III group,40.98%（25/61）,comparison between 4 groups（χ₂=8.723,P <0.05）,with statistically significant.Its’ positive expression rate was 59.09%（13/22）in lymph node metastasis group,30.78%（12/39）in no lymph node metastasis group,with statistically significant（χ₂=4.665,P< 0.05）,and Wnt3 a positive expression may have no statistically significant in clinical stage,Infiltration depth,histological type,age（P>0.05）3.The positive expression rate of Lgr5 was 0%（0/19）in normal cervical tissue group,25%（3/12）in CINI group,44.44%（8/18）in CINII-III group,65.57% in group CC（40/61）,comparison between 4 groups（χ₂=28.130,P<0.05）,with statistically significant.The positive expression of Lgr5 in G1 33.33%（4/12）,G2 70%（21/30）and G3 70%（5/19）levels was statistically significant（χ₂=7.291）.The abnormal expression rates were 38.89%（7/18）and 76.74%（33/43）,in the shallow infiltration group and the deep infiltration group,with statistically significant.and the positive expression of Lgr5 in clinical stage,pathological grade,infiltration depth,histological type and age was with no statistically significant（P>0.05）.4.In CC group,for the expression of β-catenin and Wnt3 a,there were 23 cases of both positive expression,13 cases of both negative expression,have a positive relationship（r=0.321,P <0.05）.for the expression of β-catenin and Lgr5,there were 35 cases of both positive expression,10 cases of both negative expression,have a positive relationship（r=0.321,P<0.05）.for the expression of Wnt3 a and Lgr5,there were 13 cases of both positive expression,9 cases of both negative expression,with no statistically significant（P>0.05）.Conclusions:1.The abnormal expression of β-catenin,wnt 3a and Lgr5 in normal cervical tissues,CINI CINII-III and CC showed a gradual increase,suggesting that β-catenin,Wnt 3a and Lgr5 may affect the development of cervical cancer.2.Wnt3 a may be related to the occurrence of Precancerous lesions and the transfer of cervical cancer,β-catenin and Lgr5 may be related to the degree of malignancy and invasion of cervical cancer.3.There was a positive correlation between the expression of Lgr5 and β-catenin,wnt 3a and β-catenin,suggesting that the expression of Lgr5 and wnt 3a may activate the wnt signaling pathway,leading to β-catenin move to cytoplasmic / nuclear,and cause abnormal cell proliferation and the occurrence of cervical cancer.