Extraction And Acticivoes Of Phenolic And Polysaccharide In Senecio Scandens Buch.-Ham.

Senecio scandens Buch.-Ham.(SS),a chinese hebal medicine,belongs to compositae family senecio genus.It is recorded in Chinese Pharmacopoeia as "Qianliguang".In chapter one,the composition,pharmcological activities and the extraction metholds of flavone and phenolic acid were introduced.In chapter two,mechanical parameters and extraction parameters were studied by using the yields of chlorogenic acid and hyperoside as indicators,which afforded the optimal mechanically-assisted extraction condition for phenolic compounds from SS by further employing response surface methodology.The results showed that:the optimal mechanical parameters were as follows:milling ball filling degree 27.6%,milling speed 308 r/min and milling time 11.5 min,followed by single extraction in water for 10 min to give chlorogenic acid and hyperoside in(5.040±0.042)mg/g and(0.485± 0.004)mg/g yield,respectively.Compared with heat reflux extraction and decoction,mechanically-assisted extraction was more efficient for rapid extraction of phenolic compounds at low extraction temperature without using organic solvent.Besides,the yield of hyperoside was increased substantially with obvious decrease of the residual pyrrolizidine in the extracts.In chapter three,the polysaccharides which were fewed reported home and abroad were studied.CPS was extracted from the hot-water extraction of SS,and was further separated and purified by DEAE-Sepharose Fast Flow column and Sephadex-G25,G100 column.Finally,three purified polysaccharides SP2-1,SP2-1 and SP3-2 were obtained.HPLC,HPGPC,GC-MS,IR,UV etc.methods were used to study the chemical constitutes molecular weight and manosacchairdes composition.The results showed that:the average molecular weight of SP2-1,SP2-2 and SP2-3 were 267.6,253.1 and 266.6 KDa,respectively.SP2-1 was mainly composed of rhamnose,arabinose,xylose,mannose,and galactose.SP2-2 was mainly composed of rhamnose,arabinose,xylose,glucose mannose,and galactose.SP3-2 was mainly composed of arabinose,glucose and galactose.The antioxidant and immunomodulatory assaies were studied followed,and the results showed that:all three kinds of polysaccharides had good antitoxidant activities,and promoted the spleen lymphocyte proliferation and the activity of macrophages.