Comparative Study On The Toxicity Of Formaldehyde And Methanol Exposure To Kunming Mice And Cells

Formaldehyde is widely used in industrial manufactures,is a common indoor air pollutant.Acute and chronic inhaled FA have been associated with genotoxicity neurotoxicity,reproductive toxicity,immunotoxicity and so on.The US National Research Council has made it clear that formaldehyde exposure can lead to human leukemia,But the mechanism of formaldehyde-induced leukemia remains unclear.The aim of this study is to explore the mechanism of formaldehyde metabolism and its toxic mechanism from the perspective of biology,and to provide the evidence for studying the mechanism of formaldehyde to leukemia.In this study,72 Male Kunming mice were randomly divided into 6 groups.The first group was the normal saline control group,then,FA 40 mg/kg exposure group,Methanol?MeOH?40 mg/kg exposure group,FA 10 mg/kg+MeOH 30 mg/kg exposure group,FA 20mg/kg+MeOH 20 mg/kg exposure group,FA 30 mg/kg+MeOH 10 mg/kg exposure group.Kunming mice were exposed through stomach per-fusion in 7 days.After exposure,Biomarkers of oxidative stress?ROS,MDA,GSH?in liver,the concentration of formaldehyde in blood and bone marrow,the expression of FDH in blood and bone marrow were determined.The results showed that compared with the control group,oxidative damage levels are dramatically increased;Compared with FA 10 mg/kg + MeOH 30 mg/kg group,the content of ROS decreased significantly?p<0.05?in FA 30 mg/kg + MeOH 10 mg/kg group,the content of MDA decreased significantly?p<0.01?,the content of GSH significantly increased?p<0.01?.In Combined exposure group,with the increase of the concentration of formaldehyde,the oxidative damage were on the rise;Experimental results show that there is no significant increase?p>0.05?in the blood and bone marrow formaldehyde concentration after treatment.However,the relative expression of formaldehyde dehydrogenase in blood and bone marrow increased exposure group were significantly different;Compared with the FA 30 mg/kg + MeOH 10 mg/kg,the expression of formaldehyde dehydrogenase significantly increased?p<0.01?in FA 10 mg/kg +MeOH 30 mg/kg group.In combined exposure,formaldehyde dehydrogenase increases as with the increase of formaldehyde concentration.N₂a cells were exposured to different concentrations of formaldehyde and methanol.after processing for 12h,in the individual exposure group,low concentration of formaldehyde/methanol promoted cell growth.High concentration of formaldehyde/methanol inhibited cell growth.The concentration of ROS in N₂a cells was linearly correlated with the concentration of formaldehyde/methanol.Compared with the blank group,the test group was significantly increased.After repair 24 h,the formaldehyde content returned to normal levels.In the combined group,with the increase of formaldehyde concentration,the cell viability decreased,the content of ROS increased,the content of intracellular formaldehyde increased,and then decreased to normal level.Compared with the formaldehyde 175?mol/L group,the relative expression of FDH in the 175?mol/L group was significantly decreased.The expression of FDH in the combined group increased with the increase of formaldehyde concentration.Studies have shown that formaldehyde and methanol toxicity is not consistent,formaldehyde toxicity mechanism may exist in other ways.