Correlation Of CD~4 + And CD~8 + T Lymphocytes And Interleukin-17with Acute Myocardial Infarction

Objective:We studied CD4~+ and CD8~+ levels in peripheral blood from patients with acute myocardial infarction(AMI)before and after percutaneous coronary intervention(PCI).We also examined the percentage of Th17 among CD4~+ T lymphocytes to determine whether its trend changes and to evaluate the clinical significance of such changes.Methods :We studied 80 patients with coronary angiography in June 2014 to August 2015 in the department of cardiologyin the First Affiliated Hospital of Shihezi University.All patients admitted to the hospital after were given conventional coronary heart disease secondary prevention,then dividing them into the following 3 groups:(1)an acute myocardial infarction(AMI)group(n=30),(2)an unstable angina pectoris(UAP)group(n=30),(3)a control group(n=20).The percentage of peripheral blood mononuclear cells(PBMCs)from each group was measured by flow cytometry.The differences among the groups were evaluated,and their correlations with the severity of coronary atherosclerosis(modified Gensini score)were analyzed by Spearman correlation.Results:(1)the proportions of CD4~+ lymphocytes in the UAP [27.20(32.83,22.20)%] and AMI [28.80(36.48,18.95)%] groups were significantly higher than in the normal control [17.25(22.13,14.23)% ]group before treatment(mean P<0.01),while the proportions of CD8~+ lymphocytes in the UAP [15.30(18.70,9.50)%]and AMI [9.65(17.13,7.40)%] groups before treatment were significantly lower than in the normal control [22.10(28.45,17.48)%] group(mean P<0.01).(2)Before treatment,the proportions of peripheral blood Th17 cells among CD4~+ T cells in the UAP[2.43(5.04,1.55)%] and AMI [2.93(5.11,1.84)%] groups were significantly higher than in the normal control groups[1.21(1.82,0.38)%](mean P<0.01).(3)After treatment,the CD4~+/CD8~+ratio in the AMI and UAP groups increased,as the proportion of CD8~+ T lymphocytes significantly decreased(mean P<0.05).(4)We also found that the percentage of Th17 cells among CD4~+ T cells decreased significantly in the AMI and UAP groups after treatment(mean P<0.01).(5)The following results were determined from this analysis:the levels of CD4~+ T cells in peripheral blood were positively correlated with the Gensini score(r=0.608,P<0.01);the levels of CD8~+ T cells and the Gensini scores were significantly negatively correlated(r=﹣0.603,P<0.01);the CD4~+/CD8~+ratio in peripheral blood was positively correlated with the Gensini score,and the correlation was significant(r=0.805,P<0.01);and the levels of Th17 cells and Gensini scores showed a significant positive correlation(r=0.669,P<0.01).Conclusion:(1)The present study confirmed that growing coronary atherosclerotic lesions activated the immune inflammatory response and that the percentage of CD4~+ cells increased as the percentage of CD8~+ cells decreased.Furthermore,the CD4~+/CD8~+ ratio increased significantly.(2)the levels of CD4~+ and CD8~+ T lymphocytes correlate with the severity of coronary atherosclerosis.