Effect Of Probiotics TF01 On Type 2 Diabetic Mouse By Regulating Blood Glucose And Intestinal Microbiota

Diabetes is a chronic non-communicable disease with high incidence,which is seriously harmful of human health.Current statistics found that Ⅱ diabetes accounted for the proportion of the diabetic population is as high as 90%,is the main treatment and prevention methods of medication,diet,exercise and other methods.In recent years,many studies have found that the enteric microorganism has played a major role in maintaining human health and maintained a dynamic balance in the intestinal tract.However,when these balances were broken by certain factors,the intestinal flora occured disturbance which led the body suffered from various diseases In addition,more and more studies have shown that type II diabetes have been associated with intestinal micro flora.Meanwhile,probiotics shown excellent influence on metabolism of the host body,such as maintaining gastrointestinal health,balancing immune and improving the body’s glycolipids.In this study the interventions of the probiotics TF01 for diabetes were investigated.The referred probiotic TF01 was isolated from the intestinal microflora of normal people which was found in the previous study,and the bacterial strain had a significant difference between normal people andT2 D patients,who was enrichment in the intestinal tract of normal human.Firstly,6 weeks C57B/L male mice choosed as experimental objects,and were randomly divided into normal group,model group,TF01 microbial group and TF01 fermented liquid group.Secondly,after high-fat diet(HFD)4 weeks,type 2 diabetes mellitus mices were modeled by intraperitoneal injection of low dose streptozotocin(STZ).Lastly,the above mices were grouply intervened: treatment group began to be raised the stomach probiotics TF01 until the end of the experiment,and the normal group and model group were feed the stomach equal blank medium.During the experiment,morphology of mices were observed,weight and fasting blood sugar of all the mices were monitored,and fresh feces were collected.Then,the amount of glucose tolerance(OGTT)were measured in initial stage and after the treatment(executed),respectively,calculating glucose tolerance area under the curve.The determination of blood lipid levels in mices were carried,including total cholesterol(TC),triglyceride(TG),low density lipoprotein cholesterol(LDC-C),high density lipoprotein cholesterol(HDC-C).Bacterial DNA were extracted from stool samples,and sequencing was processed in 16 S rRNA V5-V4 area using the Ion torrent platform.The sequencing results were compared with the sequencing results than bacteria database,and differences of intestinal flora structure in mice groups were analysized.The results has shown as follows: type 2 diabetes in mice after building a marked increase in the weight(p<0.01)among probiotics TF01 microbial groups and TF01 fermented liquid groups.The experimental results of fasting blood glucose(FBG)show that fasting glucose values were significantly increased in the modeled mice in addition to the normal group(p<0.01)After the experiment,fasting blood sugar levels decreased significantly(p <0.01)among TF01 microbial groups and TF01 fermented liquid groups,and blood sugar levels fell slightly in model group(p < 0.05).Experimental results of oral glucose tolerance test(OGTT)proved that glucose tolerance area under curve(AUC)significantly increased(p< 0.01)excluding the normal group,after the treatment,TF01 microbial groups had glucose tolerance small area under the curve(p < 0.05),and TF01 fermented liquid group had glucose tolerance smaller area under the curve(p < 0.01).Determination results of blood lipid in mice showed levels of TG,TC,LDL-C was increased(p < 0.05)in the blood of the model group,while levels of TC,LDL-C decreased significantly(p < 0.05),and levels of HDL–C,TC were no significant difference in TF01 bacteria liquid group;inTF01 fermented liquid group(T0),levels of LDL-C decreased significantly(p < 0.01),levels of TG,TC,HDL–C leel no significant change(p > 0.05).16 S rRNA sequencing analysis demonstrated after modeling,advantage bacterium groups were by bacteroidetes bacteria into thick wall door in type 2 diabetes in mice,after treatment,compared with normal group,flora classification is more complex on the door,key link,mesh,families and genera,level in TF01 microbial groups and TF01 fermented liquid groups,emerging a large number of lactic acid bacteria,iron bacteria,Blautia bacteria and so on.The above results showed that the probiotic TF01 could effectively reduce the fasting blood glucose in type 2 diabetes in mice,had certain improvement effect on lipid levels,which lead mice intestinal flora structure became more complicated that had more conducive to the growth of probiotics.