| Objectives Through the establishment of experimental animal model of rats with longterm drinking carbonated beverage during orthodontic tooth movement,to observe changes on contents of Calcium(Ca),Phosphorous(P)and Alkaline Phosphatase(ALP)in the blood of rats in different periods;to test bone mineral density(BMD)of proximal tibia and the first molar mobile distance;to test the expression of platelet-derived growth factor-AA(PDGF-AA)in periodontal tissue of rats during orthodontic treatment at the same time.To investigate the effect of long-term drinking carbonated drinks on orthodontic tooth movement and periodontal remodeling.Objective to provide experimental basis for orthodontic treatment of patients with long-term drinking carbonated beverages.Methods 48 Female SD rats,2 months,old,210±15g,clean grade,common feeding,Animals drank water and ate food by themselves.48 rats were randomly divided into two groups: control group and experimental group,with a total of 24 rats in each group.Each group was divided into four sub-groups based on the periods of 0 days,7 days,14 days and 21 days,with 6 in each sub-group.The control group rats were given water feeding while the experimental group was given the quantitative feeding of carbonated drinks.After 3 months,the orthodontic tooth movement device was set for each group of rats.The rats were killed at the same time of the day.Abdominal aorta blood was collected to test the contents of calcium,phosphorus and alkaline phosphatase.The left tibia was got,to test the bone mineral density.The distance of mesial movement of orthodontic molar was measured.The first molar and the surrounding periodontium of the right maxillary were got to make the histologic sections.Morphological changes of rat periodontium were observe through H-E staining.After immunohistochemical staining,the expression of PDGF-AA in the periodontal tissue of the first molar was analyzed by image analysis system of IPP6.0 software.The mean optical density(MOD)of the expression of PDGFAA was statistically analyzed.Results 1 The content of Ca,ALP and BMD in the experimental group were lower than those in the control group,while the content of P in blood was higher than that in the control group;with the extension of time,the content of Ca in blood of rats in each group,the difference was statistically significant(P<0.05).2 The distance of the first molar in the experimental group was higher than that in the control group at different time,and the difference was statistically significant(P<0.05).With the extension of time,the distance between the first molars of the two groups increased gradually,and the difference was statistically significant(P<0.05).3 According to the IHC result,there were PDGF-AA positive expressions in periodontium of rats of different groups,and the main application parts were the cytoplasm of PDLF,OB and VES.4 No matter stress application or not,the expression of PDGF-AA in the periodontal tissue of the experimental group was lower than that of the normal control group(P<0.05).Stress application can result in the strengthening of PDGF-AA expression of periodontium of the tension side of rats,which achieved the peak at 7d,and then gradually lowered down,and the difference was statistically significant(P<0.05).Conclusion 1 The experimental results show that at the same time,the mesial movement distance of orthodontic tooth for rats with long-term drinking carbonated drinks are longer than those of normal rats.As time goes on,the moving distance increases.It indicates the problem of considering the force value in clinic.2 The experiment confirms that the expression of PDGF-AA of rats with long-term drinking carbonated drinks is lower than those of normal rats.The result suggests that the stimulation of bone repair and osteogenesis can be reduced,and the long-term consumption of carbonated beverages can negatively affect the remodeling of periodontal tissue. |
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