The Expression Of CD44 And CD133 In Oral Potentially Maligant Disorders And Oral Squamous Cell Carcinoma And Their Correlation With Clinical Factors

OBJECTIVE: To explore the expression of CD44 and CD133 in oral potentially malignant disorders(OPMD)and oral squamous cell carcinoma(OSCC)as well as in human dysplastic oral mucosa keratinocyte cell line(DOK)and in human oral squamous cell carcinoma cell line(CAL-27),and to analyze their correlation with clinical factors,and to investigate if CD44 and CD133 can be used as significant indexes to evaluate the potency of OPMD to progress to OSCC.METHODS: Double immunohistochemistry were used to investigate the expression of CD44 and CD133 in the spcimens of oral normal mucosa,oral lichen planus(OLP),oral leukoplakia(OLK)and OSCC.Double immunoflourescence were used to investigate the co-expression of CD44 and CD133.Immunohistochemistry and Western Blot were used to investigate the expression and conent of CD44 and CD133 in the DOK cell line and CAL-27 cell line.Clinical factors were collected to analyze the relashionship between the expression of CD44 and CD133 and the clinical factors.SPSS19.0 software was used to analyze the expression of CD44 and CD133 in human spcimens and cell lines as well as the relationship between their expession and clinical fators.RESULTS: A gradual decrease in the expression of CD44 was observed from three groups,the positive rates of CD44 were 100.00%(10/10),96.67%(58/60),71.67%(43/60)respectively.A gradual increase in the expression of CD133 was observed from oral normal mucosa to oral potentially malignant disorders to oral squamous cell carcinoma,the positive rates of CD133 were 00.00%(0/10),35.00%(21/60),63.33%(38/60)respectively.A gradual decrease in the expression of CD44 and a gradual increase of CD133 were observed from DOK cell line to CAL-27 cell line(P<0.05).Stastically sigificant difference was found between the histological types,lymph node metasis and the expression of CD44 and CD133(P<0.05),but not reached between age,sex,location of tumor,alcohol taking,tobacco taking and their expression(P>0.05).CONCLUSIONS: CD44 and CD133 can be used as significant indexes to evaluate the potency of oral potentially maligant disorders to progress to oral squamous cell carcinoma and provide early evidences for the prevention and diagnosis of oral squamous cell carcinoma.