Synthesis And Characterization Of Water-soluble And Perylene Bisimides-based Fluorescent Poly (malic Acid) For Living Cell Labeling

Biological macromolecules has been very important to the process of human life,the production and application of functional polymer in recent years has gradualy received attaches importance in the field of biology.Recent years,perylene bisimides(PBIs)have been key materials in a verirty of fields for their important features such as strong absorption in the visible region,high luminescence efficiency,high extinction coefficients and excellent photochemical stability,and found wide range application in diverse fields of current research.Although it has many excellent properties,due to its poor water solubility also limit its applications in the biological field,therefore.To improve the water solubility of PBI become an important issue to be solved.Poly(malic acid)(PMA)is a kind of polymeric material with advantages as water solubility,biocompatibility and biodegradability.Among them,the water solubility and biocompatibity are major research focus in my thesis.So,in view of this situation,polymer containing hydrophilic ester were synthesized from perylene bisimides(PBIs)and poly(malic acid)using facile and well-established direct polycondensation.The chemical component and molecular weight of the PBI-PMA polymers were characterized by 1H NMR,FTIR,and gel permeation chromatography(GPC),respectively,The photochemical characteristics in tetrahydrofuran and water for PBI-PMA were studied by ultraviolet ray absorption spectra(UV-Vis)and fluorescence emission spectra(FL).The results show that,polymers were not only soluble in polar-organic solvent but also can be dissolved in water,and showed good fluorescent properties.As adjusting the pH of PBI-PMA aqueous solution,the fluorescene intensity change regularity along with the change of pH.The polymers are non-toxic and showed good fluorescence properties in water,thus can be applied to the cell labeling,and show application prospect and potential application in the field of biomedical.In addition,the nanoparticles can be prepared by ionic crosslinking method from chitosan and PBI-PMA.And the particles size were determined by Dynamic light scattering(DLS)as 150-300 nm.The nanoparticles can be utilized as a new drug delivery carrier.