| Objective: To observe the changes of metabolites in the fecal samples of rats with chronic immobilization stress and the effect of Xiaoyaosan.To confirm the small molecule compounds of "Metabolomics" closely associated with chronic immobilization stress and the effects of Xiaoyaosan.To explore the interventional effect of Xiaoyaosan on chronic psychological stress and the mechanisms.Method: Rats were bound to a binding rack for establishing the chronic immobilization stress models with 3 hours every day for 21 days,and Xiaoyaosan was used as the interventional drugs.72 SD rats were divided into three groups randomly: control group,model group and Xiaoyaosan group.The control group rats were not stressed and fed with 0.9% Nacl.The model group rats were stressed and fed with 0.9% Nacl correspondingly.The Xiaoyaosan group rats were stressed and fed with Xiaoyaosan,3.854g/kg per day.The appearance,the body weight and the food intake of rats in each group were observed.The behavior changes of rats were examined by the open field test and high plus maze tests.The metabonomics method based on 1H Nuclear Magnetic Resonance(1H NMR)technology was used to examine the metabolism spectral changes of fecal samples.The multivariate statistical analysis was applied to look for the differences metabolites in order to determine the small molecule compounds with the "characteristics of metabonomics" closely related to chronic bondage stress and Xiaoyaosan effects.Result:1 Changes of the appearance,the weight and the food intakeThe appearance of the model rats were worse than the control rats,and the weight and food intake lower(P<0.05,P<0.01).Compared with the model rats,the appearance of the Xiaoyaosan group were better and there was an increasing tendency in the food intake and the increased weight(P<0.01).2 Results of behavioral experimentsOn the 7th day and the 21 st day of the open field test,compared with the control group rats,the total mobile distance and the central mobile distance of the model group rats in 5 minutes declined significantly(P<0.05,P<0.01).Compared with the model group rats,the xiaoyaosan group rats increased(P<0.05,P<0.01).On the 7th day and the 21 st day of the high plus maze test,compared with the control group rats,the residence time in the open-arms and the visit times of the model group rats in 5 minutes decreased(P<0.05,P<0.01),but the residence time increased while the visit times decreased in the closed-arms,especially on the 21 st day(P<0.05,P<0.01).Compared with the model group rats,on the 21 st day,the two indictors in the open-arms of Xiaoyaosan group rats increased(P<0.05),but in the closed-arms,the residence time decreased while the times increased(P<0.05).Results of behavioral experiments mean that the chronic immobilization stress lasting 21 days may trigger the psychic reaction of rats such as fear,anxious and depression.3 Results of the multivariate statistical analysis with 1H NMR spectrumThe 8 kinds of differences metabolites(Acetate、Butyrate、Glucose、Propionate、Ribose、Maltose、Valerate、Xylose)in the fecal samples of the model group and Xiaoyaosan group rats were apparently higher than the control group(P<005,P<001),and Xiaoyaosan group rats apparently higher than the model group(P<005,P<001).Conclusion:1 Xiaoyaosan has some regulatory effects on the appearance,the food intake and the body weight of rats with chronic psychological stress,and can relieve their psychic reaction such as fear and depression.2 The carbohydrates represented by Glucose,Maltose,Ribose,Xylose,and the short-chain fatty acids represented by Acetate、Butyrate、Proionate、Valerate are the small molecule compounds of "Metabolomics" closely associated with chronic psychological stress and the effects of Xiaoyaosan.3 Regulating the species and the quantity of intestinal flora to add the production of the small molecule metabolites of carbohydrates and short-chain fatty acids may be one of the interventional mechanisms of Xiaoyaosan on chronic psychological stress. |
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