Expression Of Long Non-coding RNA Related EMT Pathway In Human Gastric Cancer And Its Clinical Significance

Objective Growing evidence suggests that long non-coding RNAs(lncRNAs)may play critical roles in epithelial-to-mesenchymal transition(EMT)progress.However,the complex molecular mechanisms remain largely unclear.Method In this study,the expression levels of lncRNAs and their target genes in EMT pathway-focused was measured by lncPathTM microarray.Levels of representative lncRNAmetallothionein 1J,pseudogene(MT1JP)and mRNA-metallothionein 1M(MT1M)were then further verified by real-time quantitative reverse transcription PCR(qRT-PCR).The relationship of their levels with clinicopathological factors was evaluated in patients with gastric cancer(GC).Receiver operating characteristic curve was used analyses potential diagnostic values.MT1 JP and MT1 M were downregulated in GCs tissues from microarray analysis results,which were further confirmed by qRT-PCR.Results LncRNA Microarrays provide comprehensive insights into the expressional relationship between LncRNAs and their target genes,which will be helpful for establishing rapid connections between the LncRNA regulatory mechanisms and their biological functions.The areas under ROC curves were 0.710 and 0.657 for MT1 JP and MT1 M,respectively.The level of MT1 JP in normal tissue is significantly higher than that in precancerous lesions and early cancers.Conclusion The reduced expression of MT1 JP and MT1 M in gastric cancer tissues,its associations with tumor diamet alr,differentiation,lymphatic metastasis,distal metastasis,invasion and tumor-node-metastasis(TNM)stage,and its aberrant expression in early cancer and precancerous lesions suggest that they may be a potential biomarker for the diagnosis of GC.