The Effects Of HGF/c-Met On The Migration Ability Of Marrow Epcs In Rats And Its Mechanisms

Objective: To investigate the effect of hepatocyte growth factor(HGF)and its receptor c-Met on the migration ability of rat bone marrow-derived endothelial progenitor cells(EPCs)and its mechanism.Methods: The EPCs were separated,obtained and identified.The Ad-c-Met-EPCs were obtained by recombinant adenovirus vector mediates c-Met transfered EPCs.The expression of c-Met and proliferation capacity of each group cells were detected by Real-time PCR,Western blot,and CCK8 respectively.The migration ability of Ad-c-Met-EPCs with different concentrations of HGF were checked by Transwell system.The each group cells were processed by proper concentration of HGF,while the PBS control group and phosphatidylinositol 3-kinase inhibitor group(add HGF and 10mg/ml LY294002)were also setted.The migration ability,Akt and P-Akt of the each group cells were detected by Transwell system and Western blot.Result:(1)The results of QPCR and Western blot showed that c-Met gene and protein in Ad-c-Met-EPCs had high expression(P<0.05).(2)The c-Met gene did not have significant impact on the appreciation capacity of EPCs.(3)HGF could increased Ad-c-Met-EPCs migration with concentration increasing(0-50ng/ml).(4)The migration capacity of HGF+Ad-c-Met-EPCs and P-Akt protein were significantly higher than other groups(P<0.05).Conclusion: HGF/c-Met can remarkably increase the migration capacity of EPCs.Besides,HGF/c-Met may accelerate the migration of EPCs through PI3K/Akt signal path.