The Changes Of Non-tremor Thermogenesis About Brown Adipose Tissue In High Fat Diet-induced Obesity Resistance Mice

Background: The phenomenon that some individuals caused obesity resistant by high energy intake is prevalent in populations and different animal species,but the mechanism is still not clear.Therefore,we established the animal model of high fat diet-induced obesity(DIO)and high fat diet-induced obesity resistance(DIO-R)by used C57BL/6J mice,to investigate the effects and mechanism of non-shivering thermogenesis in brown adipose tissue(BAT)during the process of obesity resistance.Methods: Eight-week-old C57BL/6J mice were randomly assigned to low-fat(LFD)or high-fat diet(HFD).LFD,DIO and DIO-R mice were obtained after 12 weeks of feeding.The part of animals was sacrificed after acute cold stimulation test(4℃,6 hours).Related tissues were collected after sacrificed.The morphological observation of BAT and subcutaneous adipose tissue(SAT)and the m RNA expression levels of non-shivering thermogenesis-related factors(UCP1,PGC-1α and PRDM-16)were detected.Result: The m RNA expression of UCP-1 was significantly higher in BAT of DIO-R group than that LFD and DIO group(P<0.05)at room temperature and under cold stimulation,suggesting that BAT-induced non-shivering thermogenesis was strong in DIO-R mice;DIO-R group had higher levels of UCP-1 and PGC-1α m RNA levels in SAT at room temperature and under cold stimulation than other group(P < 0.05),indicating that DIO-R mice have enhanced the levels of “adipose tissue browning” in SAT of DIO-R mice.Conclusions: The date show that increased non-shivering thermogenesis in BAT and enhanced the levels of “adipose tissue browning” in SAT of DIO-R mice to maintain a healthy weight.But the mechanisms deserve further investigation.