| Objectives To observe the effect of bone marrow mesenchymal stem cells(MSCs)in the treatment of severe acute pancreatitis(SAP)with intestinal injury,and to investigate whether the mechanism is consistent with intestinal Rho A / ROCK signaling pathway and tight junction protein.And to provide experimental basis for the clinical search for the treatment of intestinal barrier injury in patients with SAP.Methods BMSCs were separated using whole bone marrow culture method.Male Sprague-Dawley(SD)rats,weighing 200-250 g,were randomly divided into sham operation(SO)group,severe acute pancreatitis(SAP6 ? 12 ? 24h)group and bone marrow-derived mesenchymal stem cells(MSCs 6?12?24h)group.SAP group and BMSCs group were further divided into three subgroups.SAP model were induced by retrograde infusing 5% sodium taurocholate into the pancreatic duct.Bone marrow mesenchymal stem cells were transplanted by femoral vein after modeling.The sham operation group only flipped the pancreas three times.The pathologic change of pancreas and intestine were determined by light microscope.The levels of TNF-??IL-1? and D-lactate were measured by Elisa.Evans blue Determinate the capillary vessel permeability in the Small Intestine Tissue.The expressions of ROCKI and Occludin protein were detected by western blot,and the mRNA were detected by RT-PCR.Result 1?BMSCs were separated using whole bone marrow culture method and expressed CD29+99.9% ?CD90+96.6%?CD34-1.0% and CD45-0.0%.2?Compared with SO group,the pathologic injury of pancreas and intestine,TNF-?,IL-1?,D-lactate and Evans blue were increased in each SAP groups(P<0.05).Compared with SAP groups,the pathologic injury of pancreas and intestine,TNF-?,IL-1?,D-lactate and Evans blue were significantly decreased in each BMSCs groups(P<0.05).3? Compared with SO group,the expressions of intestinal ROCKI protein and m RNA were up-regulated(P<0.05),and the expressions of intestinal Occludin protein and m RNA were down-regulated in each SAP groups(P<0.05).Compared with SAP groups,the expressions of intestinal ROCKI protein and m RNA were down-regulated(P<0.05),and the expressions of intestinal Occludin protein and m RNA were up-regulated in each BMSCs groups(P<0.05).Conclusion These findings suggest that BMSCs can protect intestinal barrier function against SAP,reduce the permeability and regulate the distribution and expression of Tight junction protein.The mechanism may be related to inhibit the excessive activation of ROCKI in Rho A/ROCK pathway. |
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