Early Discrimination Of Nasopharyngeal Carcinoma Based On Tissue DNA Surface Enhanced Raman Spectroscopy Analysis

Surface Enhanced Raman Spectroscopy(SERS)was firstly employed to detect DNA variations associated with the development of nasopharyngeal carcinoma(NPC).Significant SERS spectral differences between the DNA extracted from early NPC,advanced NPC and normal nasopharyngeal tissue specimens were observed at 678,729,788,1337,1421,1506,1573 cm-1,which reflects the genetic variations in NPC.Principal component analysis for early NPC discrimination yielded a diagnostic sensitivities of 83.3%,85.4% and specificities of 80.0%,97.3% between normal nasopharyngeal and NPC and early NPC and advanced NPC,respectively.In this exploratory study,we demonstrate the potential of SERS for early detection of NPC based on DNA molecular study of biopsy tissues.