Effects Of Exercisecombined With PDTC Onexpression Of Amino Acid Transporterin Liver Of Rats Fed High Fat Diet

Background and objective : The increased fasting plasma branched-chain amino acids is a significant signal of insulin resistance and type 2 diabetes,but the elevating mechanism is not clear at present.Existing research shows that exercise can reduce plasma BCAA of IR rats,while amino acid transporter is the key of accommodating plasma BCAA egress and ingress cells,wondering the influence about amino acid transporter in plasma amino acid concentrations is significant for research about the mechanism of elevated fasting plasma BCAA level with IR and T2 DM patients.Liver is a vital organ of BCAA metabolism,changes of liver amino acid transporter may effect plasma amino acid concentrations.Studies have confirmed that high fat diets cause tissue inflammation of adipose and liver,and adipose inflammation is seemingly negatively correlated with and metabolism of BCAA.Our past research shows that exercise meet the NF-κ B pathway signal,reducing inflammation in obesity/T2 DM state,improving IR state.Knowing whether exercise can improve the transport and metabolism of amino acid through reducing inflammatory signal,may provide more solutions for treating high fat diet-produced disease.This research may use high fat diet inducing insulin resistance model rats,to explore the effect of exercise combine PDTC,the NF-κB pathway catastaltic,on amino acid plasma concentrations and liver amino acid transporter.This research may use high fat diet inducing insulin resistance model rats,to explore the effect of exercise combine PDTC,the NF-κ B pathway catastaltic,on amino acid plasma concentrations and liver amino acid transporter.Materials and methods:Select 40 SDF male rats weighing between 100g-150 g and remove 10 rats with least weight growth after 1 week of normal feeding.Select 6randomly from the remaining 30 rats for normal feeding as the normal diet group(Group A).The remaining 24 rats are fed with high-fat diet for 6 weeks.Starting from the 7th week,carry out adaptive training prior to model building for one week.Then the 24 rats are randomly divided into 4 groups,each with 6 rats.The groups are named,respectively,high fat diet quiet control group(Group H),high fat diet plus exercise group(Group HE),high fat diet plus PDTC group(Group HP),high fat diet plus exercise plus PDTC group(Group HEP).For Group HE and Group HEP,carry out 4 weeks running endurance training,5 times a week.Starting from the 10 th week,Group HP and HEP rats are injected intraperitoneally for 2 weeks(dosage: 80 mg per1 kg of body weight).In the 11 th week,after fasting for 12 hours,liver tissue andabdominal aorta blood(centrifuged in 5 min)are taken after anesthesia for plasma amino acid analysis,polymerase chain reaction and western blotting.Results:1.Plasma BCAA level of high fat diet rats is remarkable increased,especially Leucine(Leu)and Isoleucine(Ile)(P<0.05);but no evident change of the expression Liver amino acid transporter SLC36A1,SLC38A2,SLC3A2,SLC7A5 mRNA of high fat diet rats(P > 0.05);content of Liver amino acid transporter SLC36A1,SLC38A2,SLC38A4,SLC7A5,SLC7A2 is remarkable reduced(P<0.05),but SLC1A4 is remarkable increased(P<0.05);kytoplasm protein I-κB of Liver in high fat diet rats is remarkable reduced(P<0.05),nucleusis protein P65 of Liver in high fat diet rats is remarkable increased(P<0.05).2.Amino acid plasma concentrations of rats of Group HE,Group HP and Group HEP is remarkable reduced(P<0.05),and the reduced range of Group HE is obvious larger than Group HP and Group HEP(P<0.05);the expression Liver amino acid transporter SLC7A5 mRNA of high fat diet rats is remarkable increased(P<0.05).3.Comparing with Group H,the content of Liver amino acid transporter SLC7A2 in high diet rats of Group HE,Group HP and Group HEP have no obvious difference(P>0.05);the content of transporter SLC36A1 of Group HE,Group HP and Group HEP is obvious increased(P<0.05);the content of transporter SLC7A5 of Group HE is obvious increased(P<0.05),increasing range of Group HE is more than Group HP and Group HEP(P>0.05);the content of transporter SLC1A4 of Group HE is obvious decreased(P<0.05),decreasing range of Group HE is more than Group HP and Group HEP(P>0.05);the content of transporter SLC38A2 of Group HE is obvious increased(P<0.05),but Group HP and Group HEP have no increased,even decreased;the content of transporter SLC38A4 of Group HP and Group HEP is obvious increased(P<0.05),but Group HE have no obvious increased(P>0.05).Conclusion:(1).High fat diet induced fatty insulin resistance will increase plasma branched-chain amino acids level,reducing content of Liver amino acid transporter SLC36A1,SLC38A2,SLC38A4,SLC7A5,SLC7A2,but increasing Liver amino acid transporter SLC1A4;(2).Exercise and inhabiting inflammation can reduce increased plasma branched-chain amino acids level in fatty insulin resistance rats,but only inhabiting inflammation can interfere the tendency of decreasing plasma branched-chain amino acids level;(3).Exercise can improve the content of protein SLC7A5 、 SLC38A2,and the effects of exercise reducing increased plasma branched-chain amino acids level in high fat diet rats is better than PDTC,meanwhile,when exercise using inflammation catastaltic at the same time will inhibit the effects of exercise reducing plasma branched-chain amino acids level.