The Differential Expression Research Of Circular RNAs In Exosomes From Serum And Urine Of Patients With Idiopathic Membranous Nephropathy

Objective:To further explore the pathogenesis of idiopathic membranous nephropathy(IMN),the technique of gene-sequencing was used to analyze the differentially expressed circular RNAs(circRNAs)in exosomes both from serum and urine of patients with IMN,which may lay the foundation for the research of circRNAs as a new class of exosome-based idiopathic membranous nephropathy diagnosis biomarkers.Method:Ten patients with idiopathic membranous nephropathy(IMN group)and ten normal controls(NC group)were recruited as experimental subjects in our research.The serum and urine were separated from each participant's peripheral venous blood and early morning urine,which the exosomes were extracted from the collected serum and urine by the ExoQuick Exosome Precipitation Solution and ultracentrifugation.Then the pure circRNAs were extracted from the exosomes with a series of enzymatic reactions.And then,the significantly differentially expressed circRNAs were picked out by the method of gene-sequencing to analyze the function of corresponding target genes.Result:Compared with normal controls,the species of circRNAs were reduced in the exosomes from serum of patients with IMN,which were mostly originated from intron gene regions;Meanwhile,a total of 89 circRNAs were significantly differentially expressed,which were also mostly derived from intron gene regions,including 49 up-regulated and 40 down-regulaed.But the species were increased in the exosomes from urine of patients with IMN compared with normal controls,andwhich were mainly originated from exon gene regions;Simultaneously,a total of 60 circRNAs were significantly differentially expressed,which were primarily belonged to intron gene regions,including 54 up-regulated and 6 down-regulaed.Compared with the circRNAs detected from urinary exosomes,a total of 59 circRNAs were significantly differentially expressed in the exosomes from serum of patients with IMN,which were also mostly originated from intron gene regions,including 32up-regulated and 27 down-regulaed.Conclusion:The significantly differential and specific expression of circ RNAs in the exosomes from serum and urine of patients with IMN were found.Such as gene MUC3 A,which originated from chr7:100550808|100551062 could be regarded as potential diagnostic biomarkers of IMN.Furthermore,these figures suggested that the significantly differentially expressed circRNAs can be used as a reference or a supplement in the research of the pathogenesis of IMN.