| Objective:High voltage can damage many tissues and organs,such as body nerves,blood,muscles,through the electric effect,electrochemical effects and electric field force and so on.The pathogenesis and physiological mechanism of high-voltage electrical burn injury has been not fully understood.Studies suggest that oxidative stress is involved in the process of high voltage on the body damage.Studies have shown that the body produce a large number of reactive oxygen species(ROS)after burns.The ROS makes the tissues and organs damage,through lipid peroxidation and resulting in oxidative stress injury with the inflammatory factors and vasoactive substances.There are many factors that cause systemic oxidative stress in hyperbaric electrical damage.Plasma superoxide dismutase(SOD)and malondialdehyde(MDA)play an important role in regulating oxidative stress.Superoxide dismutase is kind of metal oxidase,which is widely found in the organism.SOD can catalyze the disproportionation of superoxide anion radicals,and it makes the function through the removal of superoxide ions that produced by the cells.MDA is the product of lipid peroxidation.At present,there are few studies on the quantitative and qualitative changes of serum SOD and MDA in rats after hyperbaric electrical injury.In this study,the changes of serum SOD and MDA contents in rats with high-voltage electric burn were measured,and using Ulinastatin(Ulinastatin,UTI)to meddle with this process.The aim of this study is to explore the changes of serum SOD and MDA in rats after high-voltage electrical burn,and to justify effect of the oxidative stress in the development of hyperbaric electrical injury and to explore the effect of ulinastatin`s therapeutic effect in piezoelectric burns.Methods:1 Grouping design of animal experiment: In this experiment,there are 240 adult SD rats,which offered by the Animal Experimental Center of Hebei Medical University(certificate number 832457),those rats,weight291-362 g,were randomly divided into 4 groups: without high-voltage electric burn group(without electric group),high-voltage electric burn group(injury group),the group of gived the UTI therapy after the high-voltage electric burn(UTI group),group,the group of gived the brine therapy after the high-voltage electric burn(brine group),according to the randomized block design.According to the random number table method,each group was divided into 6 groups:the first 15 minutes before the injury,5 minutes after the injury,1h,2h,4h and 8h after the injury,in which group has 10 rats.2 Preparation before the experiment:(1)the rats were weighed and given the number of registration,(2)prepare the blood collection,blood collection needle and other items for the expriment,and record the experimental data,(3)according to the instructions to configure the experimental drug to achieve the required concentration of the experiment.3 High-voltage electric burn animal model preparation: 10% chloral hydrate(0.3ml/100g)solution was injected into the rat abdominal cavity to anesthesia experimental rats,then the succcessed anesthesia rats were supine fixed in the special electric shock test stable.The left upper limb and the right lower limb were removed,with the left upper limb(current inlet)and the right lower limb(current outlet)were connected to the electrode piece(1cm×1cm).The experimental transformer and the regulator wire was connected;and then turn on the power,adjust the regulator pointer,the booster output voltage to 2kV,connected to the booster output power switch,the high voltage current through the rats call Hit,sustained electric shock 3s,high-voltage electric burns model is completed,the rats after the injury were puted into the cages,respective.The without high-voltage electric burn group didn`t given the electric,and other treatment was same as the electrical injury group and UTIgroup.Within 5min we injected 5ml/kg and 1%UTI(5ml/kg)into the abdominal of rats,respectively.Each group of rats was collected the blood and separated serum on time.4 The collection and preservation of specimens:we re-narcotized the high-pressure electric burn rats.Then opened those rats chest and exposed the heart,and we extracted 6ml blood from the heart.The blood was drawn into the coagulation tube,standing 30 min.After the serum was precipitated,we put it into the centrifuge,and adjusted the speed of 3000 rpm,10 minutes,then we took the supernatant placed in Eppendorf tube at-70℃ condition for preservation.5 Index of inspection:The levels of serum SOD and MDA were measured by enzyme linked immunosorbent assay(ELISA)in six time groups of each group,respectively.6 Statistical analysis: SPSS 21.0 statistical software was used in the statistical analysis.Two-factor factorial design of the variance analysis was the Statistical methed and the multiple comparison,we used thet test of LSD.P<0.05 was considered as significant difference.Results:1 Rats SOD serum content changesThe difference of serum SOD content between the injury group and the without electric group was significant,the serum SOD content of injury group was lower than that of the without electric group(the main effect F=165.245,P<0.05),The difference of SOD contents in injury group between every time group was significant(the main effect F=16.905,P<0.05),and the contents of SOD showed a decreasing trend with time from 5min to 8h after injury,and the time of each phase was lower than that before injury(P<0.05).SOD content reached the lowest at 8h after electric injury,but the content of SOD in the injury group was still lower than that in the without electric group,at the same time.The difference of SOD content between UTI group and saline group was significant,the serum SOD content of UTI group was higher than that of saline group(the main effect F=31.043,P<0.05).The difference of SOD content in UTI group between the every time group was significant(main effect F = 62.830,P<0.05).The levels of SOD in UTI group were higher than those before injury(P<0.05)at 5min~8h after injury,and gradually decreased.2 Rats MDA serum content changesThe difference of serum MDA content between the injury group and the without electric group was significant,the serum MDA content of injury group was higher than that of the without electric group(the main effect F=1586.203,P<0.05),The difference of MDA contents in injury group between every time group was significant(the main effect F=126.552,P<0.05),and the contents of MDA showed a increasing trend with time from 5min to 8h after injury,and the time of each phase was higher than that before injury(P<0.05).The difference of MDA content between UTI group and saline group was significant,the serum MDA content of UTI group was lower than that of saline group(the main effect F = 45.326,P<0.05).The difference of MDA content in UTI group between the every time group was significant(main effect F=246.201,P<0.05).The levels of MDA in UTI group were higher than those before injury(P<0.05)at 5min~8h after injury,and gradually increased.Conclusions:High-voltage burn caused the decrease of serum SOD and the increase of MDA content,in rats,and induced oxidative stress after high voltage electric burn.After treatment with UTI,UTI could increase the content of serum SOD and decrease the content of MDA,which indicated that UTI could inhibit the oxidative stress response after high voltage electric burn and reduce the damage the injury of oxidative stress after high voltage electric burn. |
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