| Objective The purpose of this study is to know plasma Homocysteine(Hcy)levels of essential hypertension in Qinghai Tibetans,to clear whether Homocysteine levels have relationship with polymorphisms of its related metabolic enzyme gene,and to test the hypothesis that the Hcy levels and polymorphisms of MTHFR(methylenetetrahydrofolate reductase,MTHFR)A1298C gene are associated with essential hypertension in Qinghai Tibetans,so as to greatly provide the valuable evidence for the early warnings and targeted treatment for the Qinghai Tibetans with essential hypertension.Methods We chose 60 patients with essential hypertension as the EH group who were in the department of cardiovascular at Qinghai Province People’s Hospital from August 2015 to December 2016,including 34 women and 26 men with an average age of 58.97 ± 13.38 years,and 75 free of hypertension as the control group in the same period,whose age and gender were matched with the EH group,a total of 75 cases,including 40 women and 35 men with an average age of 55.58±11.22 years.Fasting venous blood was gathered and centrifuged in one hour.We measured accurately 8 μl plasma with Shenzhen AOSA AUSA 340 II-T to detect the levels of Hcy through velocity method.We referred Gentra Puregene Handbook of QIAGEN company to extract genomic DNA in the White blood cells layer.Polymerase chain enzyme restriction fragment length polymorphism(PCR-RFLP)method was used to detect the SNP of rs1801131 in MTHFRA1298 C gene.We aim to know plasma Hcy levels of essential hypertension in Qinghai Tibetans,to clear whether Homocysteine levels have relationship with polymorphisms of its related metabolic enzyme gene,and to test the hypothesis that the Hcy levels and polymorphisms of MTHFRA1298 C gene are associated with essential hypertension.Results(1)The levels of plasma Hcy in the EH group and control group were(19.02 ± 8.31)μmol / L and(15.65 ± 5.76)μmol / l respectively.The levels of plasma Hcy were of significant difference between the two groups(P <0.05).The genotypes of MTHFRA1298 C and the levels of Hcy were AA(17.42 ± 6.33)μmol / l,AC(19.47 ± 8.24)μmol / l and CC(16.45 ± 7.30)μmol / l.There was no significant difference in the levels of Hcy between the three genotypes(P = 0.233).When controlling the confounding factors such as lipids and body mass index(BMI)by multivariate logistic regression analysis,we didn’t find HHcy was associated with the the hypertension(OR = 1.567,95% CI: 0.513-4.788,P = 0.431).(2)The genotype and frequencies of rs1801131 were AA(56.67%),AC(38.33%)and CC(5.00%)in the EH group,and the locus’ s genotype and frequencies were AA(61.33%),AC(30.67%)and CC(8.00%)in the control group.The alleles and frequencies of rs1801131 were A(75.83%)and C(24.17%)in the EH group,and the locus’ s alleles and frequencies were A(76.67%)and C(23.33%)in the control group.There was no significant difference in genotypes and alleles frequencies between the two groups(Genotype: P = 0.563,allele: P = 0.873).The genotype and frequencies of rs1801131 were AA(56.67%)and AC + CC(43.33%)in the EH group,and the locus’ s genotype and frequencies were AA(61.33%)and AC + CC(38.67%)in the control group.There was no significant difference in AA and AC + CC genotype frequencies between the two groups(P = 0.583).Conclusion(1)Plasma Hcy levels of EH patients in Qinghai Tibetans are higher than those free of EH.However,hyperhomocysteinemia(HHcy)is not a risk factor for EH.(2)MTHFR A1298 C mutation has no significant effect on plasma homocysteine levels.(3)There is no obvious correlation in polymorphisms of MTHFRA1298 C gene and essential hypertension in Qinghai Tibetans. |
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