Analysis Of 25-hydroxy Vitamin D Levels In 285 Cases Left-behind Children At 3 To 6 Years Old In Yongchuan District

Objective To investigate the status and deficiency of 25-hydroxy vitamin D[25-(OH)D] in left-behind children at 3~6 years old living in Yongchuan District and to explore the correlation of vitamin D receptor(VDR)genetic polymorphism FokI and serum levels of 25(OH)D3.Methods A total of 285 left-behind children at the age of 3 to 6 living in some towns of Yongchuan District were recruited in our hospital during November 2015 to April 2016.They all took physicl examination,and their serum level of 25-(OH)D was detected by the liquid chromatography-tandem mass spectrometry.The genotypes of FokI polymorphism for VDR gene were analyzed with polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP).Result 1.The average serum level of 25-(OH)D was(19.566±5.185)ng/ml in the 285 children,with 8 cases(2.81%)sufficient,118 cases(41.40%)insufficient,151 cases(52.98%)deficient and 8cases(2.81%)severely deficient.The level was lower in the left-behind children than the non-left-behind children,though no statisticaldifference.The level was the highest in the 3 year old group,and statistical differences were observed among the different age groups(P < 0.001).2.The serum level of 25-(OH)D was lower in the children with ff genotype than those with Ff and FF genotypes,but there was no statistical difference among different genotypes groups(P>0.05).Covariance analysis indicated that there was no statistical difference in the serum level of 25-(OH)D among the different genotypes after adjustment of age(P>0.05).Conclusion The serum level of 25(OH)D is severely low in the left behind children at the age of 3 to 6 in Yongchuan District.Some measures should be implied to improve the levels.FokI polymorphisms in VDR gene have no correlation with the serum level of 25(OH)D.