Study On The Anti-cancer Material Basis Of Petroleum Ether Fraction Of Bidens Pilosa L

Bidens pilosa L.(Compositae family),has the effect of activating blood circulation to dissipate blood stasis and clearing heat and detoxication.The whole herb has been used as a folk medicine.But,there are few reports on anti-tumor active constituences in Bidens pilosa L.It’s anticancer active fractions,pharmacological effects and mechanism are still lack of research,the methods of quality control still need to be improved.For the reason,in this paper,we primary extracted and separated different polar parts of Bidens pilosa L and screening of active fraction.The chemical component of the effective parts was analyzed by GC-MS,Through the research,we aim to lay the foundation for the development of anti-cancer medicine of Bidens pilosa L.The main contents and contributions of this paper are as follows: 1 The studies of preliminary extraction,separation and antitumor activities of Bidens pilosa LFirstly,dried herb of Bidens pilosa L was extracted with 75% ethanol,The extract was dissolved in water,and extracted with petroleum,chloroform,n-butanol and ethyl acetate,respectively.Vacuum concentration,four extract fraction was obtained.Using A549,HepG-2,CNE to screen the antitumor activities in vitro of above fractions(MTT).MTT assay in vitro,shows petroleum fraction has the best antitumor effect,especially on the lung cancer cell line(A549)(IC50=52.83±0.02 μg/mL).2 Mass Spectrometric Analysis for Bidens pilosa LUsing gas chromatography-mass spectrometry(GC-MS)to analysis the chemical component of petroleum ether fraction,identified 34 components.3 Study on Pharmacodynamics of the petroleum ether extract from Bidens pilosa L in vivoUsing the transplanted tumor model of human lung cancer A549 cells in nude mice,set low(90 mg/kg),(180 mg/kg),high dose group(360 mg/kg)to study the anticancer effect in vivo.The results showed that the inhibitory effect of petroleum ether on A549 solid tumor was dose-dependent.Among them,the high dose group had the strongest inhibitory effect,reaching 50%.The results showed that petroleum ether extract of Bidens pilosa L has certain antitumor activity.4 A preliminary study on the mechanism of antitumor effect of petroleum ether extract of Bidens pilosa LWestern blot assay was used to detect the expression of apoptosis related genes,and the expression of Caspase-3,Bax and Bcl-2 protein was studied in vitro after the intervention of petroleum ether fraction in A549 cells.The results showed that the petroleum ether fraction promoted the expression of Caspase-3 and Bax protein and decreased the level of Bcl-2 protein,and the effect was dose-dependent.The results showed that the petroleum ether fraction may play anticancer effect of Bidens pilosa L by cell apoptosis way.5 Fingerprint chromatogram analysis of Bidens pilosa LStudy on fingerprint chromatogram analysis of Bidens pilosa L.Using HPLC-UV to analysis 10 batches of different origin of Bidens pilosa L and find out 11 common peaks,established the HPLC-UV spectrum of Bidens pilosa L.There are some differences between the quality of Bidens pilosa L in different areas,excepting Anhui and Guangxi origin of Bidens pilosa L,similarity of Bidens pilosa L from 8 other areas is more than 80 %.The results show that the HPLC-UV fingerprint analysis method can be used to analyze the quality of Bidens pilosa L.