A Preliminary Study On The Expression Of FoxM1 And Caveolin-1 In Hepatocellular Carcinoma And The Relationship Between Co-expression Of FoxM1 And Caveolin-1 And The EMT Of Hepatocellular Carcinoma

Aim:Hepatocellular carcinoma which is a common malignant tumor in the world has been a serious hazard to human life.However,the development of hepatocellular carcinoma mechanism is very complex,so far the pathogenesis of hepatocellular carcinoma has not yet clear.Therefore,to explore new valuable tumor markers for the diagnosis and treatment of hepatocellular carcinoma and prognosis has important significance.This study was to investigate the expression of Caveolin-1 protein and Fox M1 transcription factor in hepatocellular carcinoma and their correlation.In addition to determine the relationship and significance of the two co-expression and epithelial mesenchymal phenotype transformation.To provide new ideas for exploring the molecular pathogenesis of invasion and metastasis of hepatocellular carcinoma.Methods:This study collected 80 cases of surgical resection of liver cancer from Fujian Medical University First Affiliated Hospital of patients with hepatocellular carcinoma.The expression of Caveolin-1 protein,FoxM1 transcription factor,epithelial marker(ZO-1,E-cadherin protein)and mesenchymal marker(N-cadherin protein,Vimentin protein)in 80 cases of hepatocellular carcinoma were detected by immunohistochemical method.And the relationship between Fox M1-Caveolin-1 co-expression and EMT and clinicopathological features of hepatocellular carcinoma was analyzed by statistical software SPSS.Results:In the 80 cases of hepatocellular carcinoma,the expression of Caveolin-1 proteinin hepatocellular carcinoma was localized in the cytoplasm,including Fox M1 protein and ZO-1 protein.The expression of E-cadherin and N-cadherin were localized on the cell membrane of hepatocarcinoma cells,and the expression of Vimentin protein is localized on the cytoplasm or nucleus of hepatocarcinoma cells.paired t-test showed a positive correlation between the expression of Caveolin-1 protein and FoxM1 transcription factor,and the difference was statistically significant(P < 0.05);χ2 test analysis showed that caveolin-1 protein expression was connected with lymph node metastasis and the degree of pathological differentiation,the differences were statistically significant(P <0.05);Fox M1 transcription factor expression was connected with the degree of pathological differentiation,the difference was statistically significant(P <0.05);Spearman rank test analysis showed that the positive expression of Caveolin-1 in hepatocellular carcinoma cells was negatively correlated with the expression of epithelial marker(ZO-1 protein,E-cadherin protein)(P <0.05).But the correlation coefficient with the interstitial markers(N-cadherin protein and Vimentin protein)was greater than 0.The positive expression of Fox M1 protein in hepatocellular carcinoma cells was negatively correlated with the expression of epithelial marker(ZO-1 protein),and positively correlated with the expression of interstitial markers(N-cadherin protein and Vimentin protein),and the difference was statistically significant(P <0.05).Conclusion:The results show that Caveolin-1 protein,Fox M1 protein expression in hepatocellular carcinoma was positively correlated,and together involved in the process of hepatocellular carcinoma EMT,and are associated with the pathological differentiation of hepatocellular carcinoma.It indicates that co-expression of Caveolin-1protein and FoxM1 transcription factor is related to the occurrence and development of hepatocellular carcinoma,which may be involved in the transformation of epithelial mesodermal phenotype of hepatocellular carcinoma and promoted the progression of hepatocellular carcinoma.This new co-expression of FoxM1-Cav-1 plays a key role in EMT phenotype and hepatocellular carcinoma malignant tumors.In general,our studyprovides a new idea for the molecular mechanisms of hepatocellular carcinoma progression and metastasis.And it may serve as a promising new biological indicator for the control of hepatocellular carcinoma progression and metastasis of new therapeutic models,in-depth mechanisms for further experimental study in the future.