| Objective: To investigate the mechanism of Protective spray against rays for red light in promoting the ulcer healing on the base of established animal model of β radiation Ⅱ degree skin injury.Methods: The model of acute radiation-induced skin ulcers in rats was replicated.Treatment was applicated of Protective spray against rays for medical use after the skin ulcer appear;Control group was applicated of placebo after the skin ulcer appear.The wound healing time were observed and the parts of the ulcer tissue were taken respectively in the 0,1,2,3,4,5 week after the ulcer had formed.The expression of VEGF,SOD activity,cell ultrastructure in these tissue and SOD activity were respectively assessed by histochemistry,biochemical detection and electron microscopy.Results:(1)The wound healing time of the Treatment group was 35.1 ± 4.1 days,it was significantly shorter than the control group(48.9 ± 4.6 days).(2)The quality of fibroblasts,epithelial cells,vascular endothelial cells in treatment group was higher than the control group which were observed by light microscopy and electron microscopy,and the damagement of the ultrastructure in treatment group was less than the control group.(3)1,2,3 weeks after treatment,the VEGF integral absorbance(IA)was significantly higher than control group.There is statistical significance between the two groups(P<0.05).(4)The SOD activity in skin tissue of the treatment group was significantly higher in 1,2,3,4,5 weeks after treatment and the difference is statistically significance(P <0.05).Conclusion: Red light has a positive effect on the healing of the rats Ⅱ degree skin injury induced by β radiation. |
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